Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein.

Autor: Bianchini F; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Crivelli V; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Abernathy ME; Department of Biology, Stanford University, Stanford, CA, USA., Guerra C; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Palus M; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic.; Veterinary Research Institute, Brno, Czech Republic., Muri J; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Marcotte H; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden., Piralla A; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy., Pedotti M; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., De Gasparo R; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Simonelli L; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Matkovic M; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Toscano C; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Biggiogero M; Clinical Research Unit, Clinica Luganese Moncucco, Lugano, Switzerland., Calvaruso V; Clinical Research Unit, Clinica Luganese Moncucco, Lugano, Switzerland., Svoboda P; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic.; Veterinary Research Institute, Brno, Czech Republic.; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.; Department of Pharmacology and Pharmacy, Faculty of Veterinary Medicine, University of Veterinary Sciences, Brno, Czech Republic., Cervantes Rincón T; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Fava T; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Podešvová L; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Shanbhag AA; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Celoria A; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Sgrignani J; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Stefanik M; Veterinary Research Institute, Brno, Czech Republic.; Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic., Hönig V; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic.; Veterinary Research Institute, Brno, Czech Republic., Pranclova V; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic.; Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic., Michalcikova T; Czech Centre of Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Vestec, Czech Republic., Prochazka J; Czech Centre of Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Vestec, Czech Republic., Guerrini G; European Commission, Joint Research Centre (JRC), Ispra Italy., Mehn D; European Commission, Joint Research Centre (JRC), Ispra Italy., Ciabattini A; Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, Siena, Italy., Abolhassani H; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.; Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran., Jarrossay D; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Uguccioni M; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Medaglini D; Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, Siena, Italy., Pan-Hammarström Q; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden., Calzolai L; European Commission, Joint Research Centre (JRC), Ispra Italy., Fernandez D; Sarafan ChEM-H Macromolecular Structure Knowledge Center, Stanford University, Stanford, CA, USA., Baldanti F; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.; Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy., Franzetti-Pellanda A; Clinical Research Unit, Clinica Luganese Moncucco, Lugano, Switzerland., Garzoni C; Internal Medicine and Infectious Diseases, Clinica Luganese Moncucco, Lugano, Switzerland., Sedlacek R; Czech Centre of Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Vestec, Czech Republic., Ruzek D; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic.; Veterinary Research Institute, Brno, Czech Republic.; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic., Varani L; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland., Cavalli A; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.; Swiss Institute of Bioinformatics, Lausanne, Switzerland., Barnes CO; Department of Biology, Stanford University, Stanford, CA, USA.; Chan Zuckerberg Biohub, San Francisco, CA, USA., Robbiani DF; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
Jazyk: angličtina
Zdroj: Science immunology [Sci Immunol] 2023 Mar 17; Vol. 8 (81), pp. eade0958. Date of Electronic Publication: 2023 Mar 10.
DOI: 10.1126/sciimmunol.ade0958
Abstrakt: Emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants diminishes the efficacy of vaccines and antiviral monoclonal antibodies. Continued development of immunotherapies and vaccine immunogens resilient to viral evolution is therefore necessary. Using coldspot-guided antibody discovery, a screening approach that focuses on portions of the virus spike glycoprotein that are both functionally relevant and averse to change, we identified human neutralizing antibodies to highly conserved viral epitopes. Antibody fp.006 binds the fusion peptide and cross-reacts against coronaviruses of the four genera, including the nine human coronaviruses, through recognition of a conserved motif that includes the S2' site of proteolytic cleavage. Antibody hr2.016 targets the stem helix and neutralizes SARS-CoV-2 variants. Antibody sd1.040 binds to subdomain 1, synergizes with antibody rbd.042 for neutralization, and, similar to fp.006 and hr2.016, protects mice expressing human angiotensin-converting enzyme 2 against infection when present as a bispecific antibody. Thus, coldspot-guided antibody discovery reveals donor-derived neutralizing antibodies that are cross-reactive with Orthocoronavirinae, including SARS-CoV-2 variants.
Databáze: MEDLINE