Biocompatibility of Veratric Acid-Encapsulated Chitosan/Methylcellulose Hydrogel: Biological Characterization, Osteogenic Efficiency with In Silico Molecular Modeling.

Autor: Durairaj K; Department of Environmental Science, School of Life Sciences, Periyar University, Salem, 636 011, India. kmdurairaj@gmail.com.; Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 54538, Iksan, Republic of Korea. kmdurairaj@gmail.com., Balasubramanian B; Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul, 05006, South Korea., Arumugam VA; Department of Human Genetics and Molecular Biology, Bharathiar University, Coimbatore- 641 046, Tamil Nadu, India., Easwaran M; Computational Biology Lab, Department of Bioinformatics, Bharathiar University, Coimbatore-46, Tamil Nadu, India, 641046., Park S; Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul, 05006, South Korea., Issara U; Division of Food Science and Technology Management, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Khlong Hok, 12110, Thailand., Pushparaj K; Department of Zoology, School of Biosciences, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, 641 043, Tamil Nadu, India., Al-Dhabi NA; Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia., Arasu MV; Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia., Liu WC; Department of Animal Science, College of Agriculture, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, People's Republic of China., Mousavi Khaneghah A; Department of Fruit and Vegetable Product Technology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology - State Research Institute, 36 Rakowiecka St., 02-532, Warsaw, Poland. amin.mousavi@ibprs.pl.; Department of Technology of Chemistry, Azerbaijan State Oil and Industry University, Baku, Azerbaijan. amin.mousavi@ibprs.pl.
Jazyk: angličtina
Zdroj: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2023 Jul; Vol. 195 (7), pp. 4429-4446. Date of Electronic Publication: 2023 Jan 26.
DOI: 10.1007/s12010-023-04311-5
Abstrakt: The limitations of graft material, and surgical sites for autografts in bone defects treatment have become a significant challenge in bone tissue engineering. Phytocompounds markedly affect bone metabolism by activating the osteogenic signaling pathways. The present study investigated the biocompatibility of the bio-composite thermo-responsive hydrogels consisting of chitosan (CS), and methylcellulose (MC) encapsulated with veratric acid (VA) as a restorative agent for bone defect treatment. The spectroscopy analyses confirmed the formation of CS/MC hydrogels and VA encapsulated CS/MC hydrogels (CS/MC-VA). Molecular analysis of the CS-specific MC decamer unit with VA complex exhibited a stable integration in the system. Further, Runx2 (runt-related transcription factor 2) was found in the docking mechanism with VA, indicating a high binding affinity towards the functional site of the Runx2 protein. The formulated CS/MC-VA hydrogels exhibited biocompatibility with the mouse mesenchymal stem cells, while VA promoted osteogenic differentiation in the stem cells, which was verified by calcium phosphate deposition through the von Kossa staining. The study results suggest that CS/MC-VA could be a potential therapeutic alternative source for bone regeneration.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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