Evaluation Expression of the Caspase-3 and Caspase-9 Apoptotic Genes in Schizophrenia Patients.

Autor:	Dirican E; Health Services Vocational School, Bayburt University, Bayburt, Turkey., Özcan H; Department of Mental Health and Related Disorders, Faculty of Medicine, Atatürk University, Erzurum, Turkey., Karabulut Uzunçakmak S; Health Services Vocational School, Bayburt University, Bayburt, Turkey., Takım U; Department of Mental Health and Related Disorders, Faculty of Medicine, Atatürk University, Erzurum, Turkey.
Jazyk:	angličtina
Zdroj:	Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology [Clin Psychopharmacol Neurosci] 2023 Feb 28; Vol. 21 (1), pp. 171-178.
DOI:	10.9758/cpn.2023.21.1.171
Abstrakt:	Objective: Apoptosis is programmed cell death that occurs by several pathways. Caspase-3 is induced by active caspase-9 via the intrinsic pathway. The aim of this research was to explore the expression of caspase-3 and caspase-9 in schizophrenia patients and healthy samples. Methods: RNA was isolated from the peripheral blood of 39 schizophrenia patients' and healthy samples. After cDNA synthesis, real time PCR (RT-PCR) was used to analyse caspase-3 and caspase-9 gene expression. The severity of psychopathological symptoms of schizophrenia was evaluated using the Positive and Negative Symptoms Scale for schizophrenia (PANSS) and Clinical Global Impressions (CGI). Results: The expression of caspase-3 and caspase-9 genes was higher in schizophrenia patients than in healthy samples ( p = 0.012, p = 0.002, respectively). The increase in caspase-3 gene expression was significant with being male, smoking and with a duration of less than 6 years ( p = 0.047, p = 0.049, p = 0.034, respectively). On the other hand, the increase in caspase-9 gene expression was significant in patients who is smoke, have children, and are under 33 years old ( p = 0.040, p = 0.043, p = 0.045, respectively). A significant positive correlation was detected between the caspase-3 and caspase-9 gene expression (r = 0.3218, p = 0.049). Conclusion: Our findings indicate that caspase-3 and caspase-9 gene expression may activate cell death mechanisms by intrinsic apoptotic genes. Furthermore, caspase-3 and caspase-9 may play essential roles in different ways in schizophrenia. Hence there is a need to further study the apoptotic mechanism with expanded patient populations.
Databáze:	MEDLINE
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