Decellularized cryopreserved human pericardium: a validation study towards tissue bank practice.

Autor: Montagner G; Fondazione Banca Dei Tessuti del Veneto Onlus, Treviso, Italy. gmontagner@fbtv-treviso.org., Barbazza A; Fondazione Banca Dei Tessuti del Veneto Onlus, Treviso, Italy., Lugas AT; PolitoBIOMed Lab, Politecnico Di Torino, Turin, Italy.; Department of Mechanical and Aerospace Engineering, Politecnico Di Torino, Turin, Italy., Terzini M; PolitoBIOMed Lab, Politecnico Di Torino, Turin, Italy.; Department of Mechanical and Aerospace Engineering, Politecnico Di Torino, Turin, Italy., Serino G; PolitoBIOMed Lab, Politecnico Di Torino, Turin, Italy.; Department of Mechanical and Aerospace Engineering, Politecnico Di Torino, Turin, Italy., Bignardi C; PolitoBIOMed Lab, Politecnico Di Torino, Turin, Italy.; Department of Mechanical and Aerospace Engineering, Politecnico Di Torino, Turin, Italy., Cacciatore M; Unità Operativa Complessa Anatomia Patologica, AULSS2 Marca Trevigiana, Ospedale Di Treviso, Treviso, Italy., Vida VL; Pediatric and Congenital Cardiac Surgery Unit, Department of Cardiac, Thoracic and Vascular Sciences and Public Health, University of Padua, Padua, Italy., Padalino MA; Pediatric and Congenital Cardiac Surgery Unit, Department of Cardiac, Thoracic and Vascular Sciences and Public Health, University of Padua, Padua, Italy., Trojan D; Fondazione Banca Dei Tessuti del Veneto Onlus, Treviso, Italy.
Jazyk: angličtina
Zdroj: Cell and tissue banking [Cell Tissue Bank] 2024 Jun; Vol. 25 (2), pp. 401-410. Date of Electronic Publication: 2023 Jan 25.
DOI: 10.1007/s10561-023-10072-6
Abstrakt: Pericardial patches are currently used as reconstructive material in cardiac surgery for surgical treatment of cardiac septal defects. Autologous pericardial patches, either treated with glutaraldehyde or not, can be used as an alternative to synthetic materials or xenograft in congenital septal defects repair. The availability of an allogenic decellularized pericardium could reduce complication during and after surgery and could be a valid alternative. Decellularization of allogenic tissues aims at reducing the immunogenic reaction that might trigger inflammation and tissue calcification over time. The ideal graft for congenital heart disease repair should be biocompatible, mechanically resistant, non-immunogenic, and should have the ability to growth with the patients. The aim of the present study is the evaluation of the efficacy of a new decellularization protocol of homologous pericardium, even after cryopreservation. The technique has proven to be suitable as a tissue bank procedure and highly successful in the removal of cells and nucleic acids content, but also in the preservation of collagen and biomechanical properties of the human pericardium.
(© 2023. The Author(s).)
Databáze: MEDLINE
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