Correlation of the DNA Concentration of Human Samples to Electrical Bioimpedance Measurements: A Pilot Study.

Autor:	Hernández-Salinas CA; Centro Militar de Ciencias de la Salud - Escuela Militar de Graduados de Sanidad, UDEFA, CDMX, México., Corzo-Cruz A; Centro Militar de Ciencias de la Salud - Escuela Militar de Graduados de Sanidad, UDEFA, CDMX, México.; Instituto Politécnico Nacional - Escuela Superior de Medicina, CDMX, México., Sánchez-Monroy V; Instituto Politécnico Nacional - Escuela Superior de Medicina, CDMX, México., Munguía-Cervantes JE; Instituto Politécnico Nacional - Centro de Nanociencias y Micro Nanotecnologías, CDMX, México., González-Díaz CA; Instituto Politécnico Nacional - Escuela Superior de Medicina, CDMX, México.
Jazyk:	angličtina
Zdroj:	Journal of electrical bioimpedance [J Electr Bioimpedance] 2023 Jan 14; Vol. 13 (1), pp. 132-135. Date of Electronic Publication: 2023 Jan 14 (Print Publication: 2022).
DOI:	10.2478/joeb-2022-0018
Abstrakt:	It is necessary to evaluate the total deoxyribonucleic acid (DNA) concentration in gene expression assays. The existing techniques require equipment that is expensive for many labs in developing countries. Portable and inexpensive equipment is needed for easy and economical DNA quantification. Electrical bioimpedance spectroscopy (EBiS) is a non-invasive and inexpensive technique for examining the electrical properties of biological materials. The aim of this study was to explore a potential correlation between the measurement of total DNA extracted from human samples by UV-Vis spectrophotometry and EBiS. Hence, after quantifying the total DNA extracted from each sample by UV-Vis spectroscopy, EBiS was recorded and a possible correlation between the two measurements was analyzed. Considering the bioimpedance phase parameter at 5.24 MHz, a significant correlation was found with total DNA, especially when the concentration was below 100 ng/μL (Spearman coefficient = 0.82, p<0.005). Additional experiments are warranted to confirm these findings. Competing Interests: Conflict of interest The authors declare that they have no conflict of interest. (© 2022 César A. Hernández-Salinas, Alejandro Corzo-Cruz, Virginia Sánchez-Monroy, Jacobo E. Munguía-Cervantes, César A. González-Díaz, published by Sciendo.)
Databáze:	MEDLINE
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