Deciphering the biodesulfurization potential of two novel Rhodococcus isolates from a unique Greek environment.

Autor:	Glekas PD; Enzyme and Microbial Biotechnology Unit, Department of Biology, National and Kapodistrian University of Athens, Zografou Campus, 15784 Athens, Greece., Martzoukou O; Enzyme and Microbial Biotechnology Unit, Department of Biology, National and Kapodistrian University of Athens, Zografou Campus, 15784 Athens, Greece., Mastrodima ME; Enzyme and Microbial Biotechnology Unit, Department of Biology, National and Kapodistrian University of Athens, Zografou Campus, 15784 Athens, Greece., Zarkadoulas E; Enzyme and Microbial Biotechnology Unit, Department of Biology, National and Kapodistrian University of Athens, Zografou Campus, 15784 Athens, Greece., Kanakoglou DS; Department of Biological Chemistry, Medical School, National and Kapodistrian University of Athens, 75 Mikras Asias Street, 11527 Athens, Greece., Kekos D; Biotechnology Laboratory, Sector of Synthesis and Development of Industrial Processes (IV), School of Chemical Engineering, National Technical University of Athens, Athens, Greece., Pachnos M; Division of European Affairs, Motor Oil Hellas, 15121 Marousi, Athens, Greece., Mavridis G; Division of European Affairs, Motor Oil Hellas, 15121 Marousi, Athens, Greece., Mamma D; Biotechnology Laboratory, Sector of Synthesis and Development of Industrial Processes (IV), School of Chemical Engineering, National Technical University of Athens, Athens, Greece., Hatzinikolaou DG; Enzyme and Microbial Biotechnology Unit, Department of Biology, National and Kapodistrian University of Athens, Zografou Campus, 15784 Athens, Greece.
Jazyk:	angličtina
Zdroj:	AIMS microbiology [AIMS Microbiol] 2022 Dec 15; Vol. 8 (4), pp. 484-506. Date of Electronic Publication: 2022 Dec 15 (Print Publication: 2022).
DOI:	10.3934/microbiol.2022032
Abstrakt:	Sustainable biodesulfurization (BDS) processes require the use of microbial biocatalysts that display high activity against the recalcitrant heterocyclic sulfur compounds and can simultaneously withstand the harsh conditions of contact with petroleum products, inherent to any industrial biphasic BDS system. In this framework, the functional microbial BDS-related diversity in a naturally oil-exposed ecosystem, was examined through a 4,6-dimethyl-dibenzothiophene based enrichment process. Two new Rhodococcus sp. strains were isolated, which during a medium optimization process revealed a significantly enhanced BDS activity profile when compared to the model strain R. qingshengii IGTS8. In biocatalyst stability studies conducted in biphasic mode using partially hydrodesulfurized diesel under various process conditions, the new strains also presented an enhanced stability phenotype. In these studies, it was also demonstrated for all strains, that the BDS activity losses were decoupled from the overall cells' viability, in addition to the fact that the use of whole-broth biocatalyst positively affected BDS performance. Competing Interests: Conflict of interest: The authors declare no conflict of interest. (© 2022 the Author(s), licensee AIMS Press.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu