Human adipose mesenchymal stromal cells growing into PCL-nHA electrospun scaffolds undergo hypoxia adaptive ultrastructural changes.

Autor: Frontini-López YR; IHEM, Universidad Nacional de Cuyo, CONICET, Mendoza, Argentina.; Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Buenos Aires, Argentina., Rivera L; IHEM, Universidad Nacional de Cuyo, CONICET, Mendoza, Argentina., Aldana AA; Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA, UNMdP-CONICET), Mar del Plata, Argentina., Rivero G; Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA, UNMdP-CONICET), Mar del Plata, Argentina., Liverani L; Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany., Abraham GA; Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA, UNMdP-CONICET), Mar del Plata, Argentina., Boccaccini AR; Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany., Bustos DM; IHEM, Universidad Nacional de Cuyo, CONICET, Mendoza, Argentina.; Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Mendoza, Argentina., Uhart M; IHEM, Universidad Nacional de Cuyo, CONICET, Mendoza, Argentina.
Jazyk: angličtina
Zdroj: Biotechnology journal [Biotechnol J] 2023 Apr; Vol. 18 (4), pp. e2200413. Date of Electronic Publication: 2023 Feb 02.
DOI: 10.1002/biot.202200413
Abstrakt: Human Adipose-Derived Mesenchymal Stem/Stromal Cells (hAD-MSCs) have great potential for tissue regeneration. Since transplanted hAD-MSCs are likely to be placed in a hypoxic environment, culturing the cells under hypoxic conditions might improve their post-transplantation survival and regenerative performance. The combination of hAD-MSCs and PCL-nHA nanofibers synergically improves the contribution of both components for osteoblast differentiation. In this work, we hypothesized that this biomaterial constitutes a hypoxic environment for hAD-MSCs. We studied the cellular re-arrangement and the subcellular ultrastructure by Transmission Electron Microscopy (TEM) of hAD-MSCs grown into PCL-nHA nanofibers, and we compared them with the same cells grown in two-dimensional cultures, over tissue culture-treated plastic, or glass coverslips. Among the most evident changes, PCL-nHA grown cells showed enlarged mitochondria, and accumulation of glycogen granules, consistent with a hypoxic environment. We observed a 3.5 upregulation (p = 0.0379) of Hypoxia Inducible Factor (HIF)-1A gene expression in PCL-nHA grown cells. This work evidences for the first time intra-cellular changes in three-dimensional compared to two-dimensional cultures, which are adaptive responses of the cells to an environment more closely resembling that of the in vivo niche after transplantation, thus PCL-nHA nanofibers are adequate for hAD-MSCs pre-conditioning.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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