3D RNA-scaffolded wireframe origami.

Autor: Parsons MF; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Allan MF; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Microbiology, Harvard Medical School, Boston, MA, USA.; Computational and Systems Biology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Li S; Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA.; MOE Key Laboratory for Cellular Dynamics and Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, China., Shepherd TR; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Inscripta, Inc., Boulder, CO, 80027, USA., Ratanalert S; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA., Zhang K; Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA.; MOE Key Laboratory for Cellular Dynamics and Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, China., Pullen KM; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Chiu W; Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA.; CryoEM and Bioimaging Division, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Stanford University, Menlo Park, CA, 94025, USA., Rouskin S; Department of Microbiology, Harvard Medical School, Boston, MA, USA., Bathe M; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. mark.bathe@mit.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Jan 24; Vol. 14 (1), pp. 382. Date of Electronic Publication: 2023 Jan 24.
DOI: 10.1038/s41467-023-36156-1
Abstrakt: Hybrid RNA:DNA origami, in which a long RNA scaffold strand folds into a target nanostructure via thermal annealing with complementary DNA oligos, has only been explored to a limited extent despite its unique potential for biomedical delivery of mRNA, tertiary structure characterization of long RNAs, and fabrication of artificial ribozymes. Here, we investigate design principles of three-dimensional wireframe RNA-scaffolded origami rendered as polyhedra composed of dual-duplex edges. We computationally design, fabricate, and characterize tetrahedra folded from an EGFP-encoding messenger RNA and de Bruijn sequences, an octahedron folded with M13 transcript RNA, and an octahedron and pentagonal bipyramids folded with 23S ribosomal RNA, demonstrating the ability to make diverse polyhedral shapes with distinct structural and functional RNA scaffolds. We characterize secondary and tertiary structures using dimethyl sulfate mutational profiling and cryo-electron microscopy, revealing insight into both global and local, base-level structures of origami. Our top-down sequence design strategy enables the use of long RNAs as functional scaffolds for complex wireframe origami.
(© 2023. The Author(s).)
Databáze: MEDLINE
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