Effect of culture conditions at lab-scale on metabolite composition and antibacterial and antibiofilm activities of Dunaliella tertiolecta.

Autor: Iglesias MJ; Área de Química Orgánica, Research Centre CIAIMBITAL, Universidad de Almería, Almería, Spain., Soengas R; Área de Química Orgánica, Research Centre CIAIMBITAL, Universidad de Almería, Almería, Spain., López-Ortiz F; Área de Química Orgánica, Research Centre CIAIMBITAL, Universidad de Almería, Almería, Spain., Biondi N; Department of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, Florence, Italy., Tredici MR; Department of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, Florence, Italy., Gutiérrez-Del-Río I; Área de Microbiología, Research Group BIONUC, Universidad de Oviedo, IUOPA (Instituto Universitario de Oncología del Principado de Asturias), ISPA (Instituto de Investigación Sanitaria del Principado de Asturias), Oviedo, Spain., López-Ibáñez S; Área de Microbiología, Research Group BIONUC, Universidad de Oviedo, IUOPA (Instituto Universitario de Oncología del Principado de Asturias), ISPA (Instituto de Investigación Sanitaria del Principado de Asturias), Oviedo, Spain., Villar CJ; Área de Microbiología, Research Group BIONUC, Universidad de Oviedo, IUOPA (Instituto Universitario de Oncología del Principado de Asturias), ISPA (Instituto de Investigación Sanitaria del Principado de Asturias), Oviedo, Spain., Lombó F; Área de Microbiología, Research Group BIONUC, Universidad de Oviedo, IUOPA (Instituto Universitario de Oncología del Principado de Asturias), ISPA (Instituto de Investigación Sanitaria del Principado de Asturias), Oviedo, Spain., López Y; ISGlobal, Hospital Clínic-Universitat de Barcelona, Barcelona, Spain., Gabasa Y; ISGlobal, Hospital Clínic-Universitat de Barcelona, Barcelona, Spain., Soto S; ISGlobal, Hospital Clínic-Universitat de Barcelona, Barcelona, Spain.
Jazyk: angličtina
Zdroj: Journal of phycology [J Phycol] 2023 Apr; Vol. 59 (2), pp. 356-369. Date of Electronic Publication: 2023 Mar 09.
DOI: 10.1111/jpy.13316
Abstrakt: Dunaliella tertiolecta RCC6 was cultivated indoors in glass bubble column photobioreactors operated under batch and semi-continuous regimens and using two different conditions of light and temperature. Biomass was harvested by centrifugation, frozen, and then lyophilized. The soluble material was obtained by sequential extraction of the lyophilized biomass with solvents with a gradient of polarity (hexane, ethyl acetate, and methanol) and its metabolic composition was investigated through nuclear magnetic resonance (NMR) spectroscopy. The effect of light on chlorophyll biosynthesis was clearly shown through the relative intensities of the 1 H NMR signals due to pheophytins. The highest signal intensity was observed for the biomasses obtained at lower light intensity, resulting in a lower light availability per cell. Under high temperature and light conditions, the 1 H NMR spectra of the hexane extracts showed an incipient accumulation of triacylglycerols. In these conditions and under semi-continuous regimen, an enhancement of β-carotene and sterols production was observed. The antibacterial and antibiofilm activities of the extracts were also tested. Antibacterial activity was not detected, regardless of culture conditions. In contrast, the minimal biofilm inhibitory concentrations (MBICs) against Escherichia coli for the hexane extract obtained under semi-continuous regimen using high temperature and irradiance conditions was promising.
(© 2023 Phycological Society of America.)
Databáze: MEDLINE
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