Effects of Conditioned Medium of Adipose-Derived Stem Cells Exposed to Platelet-Rich Plasma on the Expression of Endothelial Nitric Oxide Synthase and Angiogenesis by Endothelial Cells.

Autor:	Morita M; From the Departments of Plastic and Reconstructive Surgery., Suyama Y; From the Departments of Plastic and Reconstructive Surgery., Notsu T; Genomic Medicine and Regenerative Therapy., Fukuoka K; From the Departments of Plastic and Reconstructive Surgery., Ikuta K; From the Departments of Plastic and Reconstructive Surgery., Kanayama H; From the Departments of Plastic and Reconstructive Surgery., Umeda R; From the Departments of Plastic and Reconstructive Surgery., Teraoka S; Urology., Minato H; Anesthesiology., Ninomiya H; Biological Regulation, Tottori University Faculty of Medicine., Tsuneto M; Genomic Medicine and Regenerative Therapy., Shirayoshi Y; Genomic Medicine and Regenerative Therapy., Hisatome I, Yagi S; From the Departments of Plastic and Reconstructive Surgery.
Jazyk:	angličtina
Zdroj:	Annals of plastic surgery [Ann Plast Surg] 2023 Feb 01; Vol. 90 (2), pp. 171-179.
DOI:	10.1097/SAP.0000000000003368
Abstrakt:	Abstract: Platelet-rich plasma (PRP) and adipose-derived stem cells (ADSCs) are known to secrete angiogenic factors that contribute to the treatment of intractable ulcers. The combination of PRP and ADSCs may enhance their angiogenic effects. However, it remains unclear whether treatment of ADSCs with PRP influences angiogenesis. We studied whether the conditioned medium from PRP-treated ADSCs under hypoxic conditions exerts angiogenic effects. Although PRP stimulated the proliferation of ADSCs obtained from rats, it decreased the mRNA levels of vascular endothelial growth factor, hepatocyte growth factor, and TGF-β1, but not of basic fibroblast growth factor, under hypoxia. The conditioned medium of PRP-treated ADSCs inhibited endothelial nitric oxide synthase phosphorylation, decreased NO production, and suppressed tube formation in human umbilical vein endothelial cells. Transplantation of ADSCs alone increased both blood flow and capillary density of the ischemic limb; however, its combination with PRP did not further improve blood flow or capillary density. This suggests that both conditioned medium of ADSCs treated with PRP and combination of PRP with ADSCs transplantation may attenuate the phosphorylation of endothelial nitric oxide synthase and angiogenesis. Competing Interests: Conflicts of interest and sources of funding: None declared. (Copyright © 2022 The Author(s). Published by Wolters Kluwer Health, Inc.)
Databáze:	MEDLINE
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