Monkeypox infection elicits strong antibody and B cell response against A35R and H3L antigens.

Autor:	Yefet R; Department of Microbiology and Clinical Immunology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Friedel N; Division of Dermatology, Tel-Aviv Sourasky Medical Center, Tel Aviv, Israel., Tamir H; Department of Infectious Diseases, The Israel Institute for Biological Research, Ness Ziona, Israel., Polonsky K; Department of Microbiology and Clinical Immunology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.; Edmond J. Safra Center for Bioinformatics at Tel-Aviv University, Tel Aviv, Israel., Mor M; Department of Microbiology and Clinical Immunology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Cherry-Mimran L; Department of Infectious Diseases, The Israel Institute for Biological Research, Ness Ziona, Israel., Taleb E; Division of Dermatology, Tel-Aviv Sourasky Medical Center, Tel Aviv, Israel., Hagin D; Department of Immunology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel., Sprecher E; Division of Dermatology, Tel-Aviv Sourasky Medical Center, Tel Aviv, Israel.; Department of Human Molecular Genetics and Biochemistry, Faculty of Medicine, Tel-Aviv University, Tel Aviv, Israel., Israely T; Department of Infectious Diseases, The Israel Institute for Biological Research, Ness Ziona, Israel., Freund NT; Department of Microbiology and Clinical Immunology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2023 Feb 17; Vol. 26 (2), pp. 105957. Date of Electronic Publication: 2023 Jan 13.
DOI:	10.1016/j.isci.2023.105957
Abstrakt:	Monkeypox virus (MPXV) resides in two forms; mature and enveloped, and depending on it, distinct proteins are displayed on the viral surface. Here, we expressed two MPXV antigens from the mature, and one from the enveloped form, and tested their reactivity to sera of 11 MPXV recoverees while comparing to sera from recently and past vaccinated individuals. 8 out of 11 recoverees exhibited detectable neutralization levels against Vaccinia Lister. Sera from all recoverees bound strongly to A35R and H3L antigens. Moreover, the responses to A35R were significantly higher within the recoverees compared to both recently and past vaccinated donors. Lastly, A35R- and H3L-specific IgG + B cells ranging from 0.03-0.46% and 0.11-0.36%, respectively, were detected in all recoverees (A35R), and in 9 out of 11 recoverees (H3L). Therefore, A35R and H3L represent MPXV immune targets and could be used in a heat-inactivated serological ELISA for the identification of recent MPXV infection. Competing Interests: The authors declare no conflict of interest. (© 2023 The Author(s).)
Databáze:	MEDLINE
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