Green and cost-effective voltammetric assay for spiramycin based on activated glassy carbon electrode and its applications to urine and milk samples.

Autor:	Noureldin HAM; Department of Analytical Chemistry, Faculty of Pharmacy, Badr University in Cairo Cairo Egypt., Abdel-Aziz AM; Department of Chemistry, Faculty of Science, Ain Shams University Cairo Egypt ihbadr@sci.asu.edu.eg., Mabrouk MM; Department of Analytical Chemistry, Faculty of Pharmacy, Tanta University Tanta Egypt., Saad AHK; Department of Analytical Chemistry, Faculty of Pharmacy, Tanta University Tanta Egypt., Badr IHA; Department of Chemistry, Faculty of Science, Ain Shams University Cairo Egypt ihbadr@sci.asu.edu.eg.; Department of Chemistry, Faculty of Science, Galala University Suez Egypt ibrahim.badr@gu.edu.eg.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2023 Jan 03; Vol. 13 (2), pp. 844-852. Date of Electronic Publication: 2023 Jan 03 (Print Publication: 2023).
DOI:	10.1039/d2ra06768d
Abstrakt:	A simple, cost-effective, and efficient differential pulse voltammetric (DPV) assay for monitoring spiramycin adipate (SPA) in its dosage forms, urine, and milk samples at an activated glassy carbon electrode (GCE) was developed. GCE was electrochemically activated by anodization at a high positive voltage (2.5 V). The activated glassy carbon electrode (AGCE) was surface characterized, optimized, and utilized for the electrochemical assay of SPA. The electrochemical behavior of the AGCEs was investigated using cyclic voltammetry (CV) which shows a remarkable increase in the anodic peak of SPA in comparison with GCE. This behavior reflects a remarkable increase in the electrocatalytic oxidation of SPA at AGCE. The impacts of various parameters such as scan rate, accumulation time, and pH were investigated. The analytical performance of the activated glassy carbon electrodes was studied utilizing DPV. Under optimum conditions, the oxidation peak current exhibited two linear ranges of 80 nm to 0.8 μM and 0.85-300 μM with a lower limit of detection (LOD) of 20 nM. The developed assay exhibited high sensitivity, excellent repeatability, and good selectivity. Additionally, the developed SPA-sensitive modified GCE was successfully applied for SPA assay in its pharmaceutical dosage form and diluted biological fluids as well, with satisfactory recovery results which correlated well with the results obtained using spectrophotometry. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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