Chitosan loaded RNA polymerase inhibitor nanoparticles increased attenuation in toxin release from Streptococcus pneumonia .
| Autor: | Yahya Alqahtani F; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia., Sfouq Aleanizy F; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia., Alkahtani HM; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia., El Tahir E; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia., Akber Ansari S; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia., Alharbi A; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia., Al-Bdrawy A; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia., Shakeel F; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia., Haq N; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia., Al-Rasheed LS; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia., Alfaraj R; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia., Alshememry AK; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia., Alsarra IA; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia. |
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| Jazyk: | angličtina |
| Zdroj: | Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society [Saudi Pharm J] 2023 Jan; Vol. 31 (1), pp. 170-179. Date of Electronic Publication: 2022 Nov 25. |
| DOI: | 10.1016/j.jsps.2022.11.015 |
| Abstrakt: | Background: Multidrug-resistant (MDR) bacterial infections have become an emerging health concern around the world. Antibiotics resistance among S. pneumoniae strains increased recently contributing to increase in incidence of pneumococcal infection. This necessitates the discovery of novel antipnemococcal such as compound C3-005 which target the interaction between RNA polymerase and σ factors. Chitosan nanoparticles (CNPs) exhibited antibacterial activity including S. pneumonia . Therefore, the aims of the current investigation were to formulate CNPs loaded with C3-005 and characteristic their antimicrobial properties against S. pneumonia. Methods: The CNPs and C3-005 loaded CNPs were produced utilizing ionic gelation method, and their physicochemical characteristics including particle size, zeta potential, polydispersity index (PDI), encapsulation efficiency (EE%), and in vitro release profile were studied. Both differential scanning calorimetry (DSC) and fourier transform infrared spectroscopy (FTIR) were used for chemical characterization. The synthesized NPs' minimum inhibitory concentration (MIC) was determined using killing assay and broth dilution method, and their impact on bacteria induced hemolysis were also studied. Results: The NPs encapsulating C3-005 were successfully prepared with particle size of 343.5 nm ± 1.3, zeta potential of 29.8 ± 0.37, and PDI of 0.20 ± 0.03. 70 % of C3-005 were encapsulated in CNPs and sustained release pattern of C3-005 from CNPs was revealed by an in vitro release study. CNPs containing C3-005 exhibited higher antipnomcoccal activity with MIC Conclusions: The findings of this study showed the potential for using C3-005 loaded CNPs to treat pneumococcal infection. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2022 The Author(s).) |
| Databáze: | MEDLINE |
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