Selectivity and stability of N-terminal targeting protein modification chemistries.

Autor:	Barber LJ; Department of Chemistry, University of York Heslington YO10 5DD UK chris.spicer@york.ac.uk.; York Biomedical Research Institute, University of York Heslington YO10 5DD UK.; Department of Biology, University of York Heslington YO10 5DD UK., Yates NDJ; Department of Chemistry, University of York Heslington YO10 5DD UK chris.spicer@york.ac.uk.; York Biomedical Research Institute, University of York Heslington YO10 5DD UK., Fascione MA; Department of Chemistry, University of York Heslington YO10 5DD UK chris.spicer@york.ac.uk.; York Biomedical Research Institute, University of York Heslington YO10 5DD UK., Parkin A; Department of Chemistry, University of York Heslington YO10 5DD UK chris.spicer@york.ac.uk., Hemsworth GR; Astbury Centre for Structural Molecular Biology and School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds Leeds LS2 9JT UK., Genever PG; York Biomedical Research Institute, University of York Heslington YO10 5DD UK.; Department of Biology, University of York Heslington YO10 5DD UK., Spicer CD; Department of Chemistry, University of York Heslington YO10 5DD UK chris.spicer@york.ac.uk.; York Biomedical Research Institute, University of York Heslington YO10 5DD UK.; Department of Biology, University of York Heslington YO10 5DD UK.
Jazyk:	angličtina
Zdroj:	RSC chemical biology [RSC Chem Biol] 2022 Nov 17; Vol. 4 (1), pp. 56-64. Date of Electronic Publication: 2022 Nov 17 (Print Publication: 2023).
DOI:	10.1039/d2cb00203e
Abstrakt:	Protein N-termini provide uniquely reactive motifs for single site protein modification. Though a number of reactions have been developed to target this site, the selectivity, generality, and stability of the conjugates formed has not been studied. We have therefore undertaken a comprehensive comparative study of the most promising methods for N-terminal protein modification, and find that there is no 'one size fits all' approach, necessitating reagent screening for a particular protein or application. Moreover, we observed limited stability in all cases, leading to a need for continued innovation and development in the bioconjugation field. Competing Interests: The authors declare no competing financial interests. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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