Detection of West Nile Virus Lineage 2 in Eastern Romania and First Identification of Sindbis Virus RNA in Mosquitoes Analyzed using High-Throughput Microfluidic Real-Time PCR.

Autor: Crivei LA; Regional Center of Advanced Research for Emerging Diseases, Zoonoses and Food Safety, Iași University of Life Sciences, 700490 Iași, Romania., Moutailler S; ANSES, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700 Maisons-Alfort, France., Gonzalez G; ANSES, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR VIROLOGIE, Laboratoire de Santé Animale, 94700 Maisons-Alfort, France., Lowenski S; ANSES, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR VIROLOGIE, Laboratoire de Santé Animale, 94700 Maisons-Alfort, France., Crivei IC; Regional Center of Advanced Research for Emerging Diseases, Zoonoses and Food Safety, Iași University of Life Sciences, 700490 Iași, Romania., Porea D; Regional Center of Advanced Research for Emerging Diseases, Zoonoses and Food Safety, Iași University of Life Sciences, 700490 Iași, Romania., Anita DC; Regional Center of Advanced Research for Emerging Diseases, Zoonoses and Food Safety, Iași University of Life Sciences, 700490 Iași, Romania., Ratoi IA; Regional Center of Advanced Research for Emerging Diseases, Zoonoses and Food Safety, Iași University of Life Sciences, 700490 Iași, Romania., Zientara S; ANSES, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR VIROLOGIE, Laboratoire de Santé Animale, 94700 Maisons-Alfort, France., Oslobanu LE; Regional Center of Advanced Research for Emerging Diseases, Zoonoses and Food Safety, Iași University of Life Sciences, 700490 Iași, Romania., Tomazatos A; Department of Arbovirology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany., Savuta G; Regional Center of Advanced Research for Emerging Diseases, Zoonoses and Food Safety, Iași University of Life Sciences, 700490 Iași, Romania., Lecollinet S; ANSES, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR VIROLOGIE, Laboratoire de Santé Animale, 94700 Maisons-Alfort, France.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2023 Jan 09; Vol. 15 (1). Date of Electronic Publication: 2023 Jan 09.
DOI: 10.3390/v15010186
Abstrakt: The impact of mosquito-borne diseases on human and veterinary health is being exacerbated by rapid environmental changes caused mainly by changing climatic patterns and globalization. To gain insight into mosquito-borne virus circulation from two counties in eastern and southeastern Romania, we have used a combination of sampling methods in natural, urban and peri-urban sites. The presence of 37 mosquito-borne viruses in 16,827 pooled mosquitoes was analyzed using a high-throughput microfluidic real-time PCR assay. West Nile virus (WNV) was detected in 10/365 pools of Culex pipiens ( n = 8), Culex modestus ( n = 1) and Aedes vexans ( n = 1) from both studied counties. We also report the first molecular detection of Sindbis virus (SINV) RNA in the country in one pool of Culex modestus . WNV infection was confirmed by real-time RT-PCR (10/10) and virus isolation on Vero or C6/36 cells (four samples). For the SINV-positive pool, no cytopathic effectwas observed after infection of Vero or C6/36 cells, but no amplification was obtained in conventional SINV RT-PCR. Phylogenetic analysis of WNV partial NS5 sequences revealed that WNV lineage 2 of theCentral-Southeast European clade, has a wider circulation in Romania than previously known.
Databáze: MEDLINE
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