| Autor: |
Molendijk MM; Department Medical Microbiology and Infectious Diseases, Erasmus MC, 3015 Rotterdam, The Netherlands.; Department of Viroscience, Erasmus MC, 3015 Rotterdam, The Netherlands., Phan MVT; Department of Viroscience, Erasmus MC, 3015 Rotterdam, The Netherlands.; Medical Research Council/Uganda Virus Research Institute, London School of Hygiene & Tropical Medicine Uganda Research Unit, Entebbe P.O. Box 49, Uganda., Bode LGM; Department Medical Microbiology and Infectious Diseases, Erasmus MC, 3015 Rotterdam, The Netherlands., Strepis N; Department Medical Microbiology and Infectious Diseases, Erasmus MC, 3015 Rotterdam, The Netherlands., Prasad DK; Department of Viroscience, Erasmus MC, 3015 Rotterdam, The Netherlands., Worp N; Department of Viroscience, Erasmus MC, 3015 Rotterdam, The Netherlands., Nieuwenhuijse DF; Department of Viroscience, Erasmus MC, 3015 Rotterdam, The Netherlands., Schapendonk CME; Department of Viroscience, Erasmus MC, 3015 Rotterdam, The Netherlands., Boekema BKHL; Association of Dutch Burn Centres, 1941 AJ Beverwijk, The Netherlands., Verbon A; Department Medical Microbiology and Infectious Diseases, Erasmus MC, 3015 Rotterdam, The Netherlands., Koopmans MPG; Department of Viroscience, Erasmus MC, 3015 Rotterdam, The Netherlands., Graaf M; Department of Viroscience, Erasmus MC, 3015 Rotterdam, The Netherlands., van Wamel WJB; Department Medical Microbiology and Infectious Diseases, Erasmus MC, 3015 Rotterdam, The Netherlands. |
| Abstrakt: |
Infections involving antibiotic resistant Staphylococcus aureus ( S. aureus ) represent a major challenge to successful treatment. Further, although bacteriophages (phages) could be an alternative to antibiotics, there exists a lack of correlation in phage susceptibility results between conventional in vitro and in vivo assays. This discrepancy may hinder the potential implementation of bacteriophage therapy. In this study, the susceptibility of twelve S. aureus strains to three commercial phage cocktails and two single phages was assessed. These S. aureus strains (including ten clinical isolates, five of which were methicillin-resistant) were compared using four assays: the spot test, efficiency of plating (EOP), the optical density assay (all in culture media) and microcalorimetry in human serum. In the spot test, EOP and optical density assay, all cocktails and single phages lysed both methicillin susceptible and methicillin resistant S. aureus strains. However, there was an absence of phage-mediated lysis in high concentrations of human serum as measured using microcalorimetry. As this microcalorimetry-based assay more closely resembles in vivo conditions, we propose that microcalorimetry could be included as a useful addition to conventional assays, thereby facilitating more accurate predictions of the in vivo susceptibility of S. aureus to phages during phage selection for therapeutic purposes. |
