| Autor: |
Dzul-Rosado K; Dr. Hideyo Noguchi Regional Research Center, Autonomous University of Yucatan, Mérida 97000, Mexico., Donis-Maturano L; Faculty of Higher Studies-Iztacala, National Autonomous University of Mexico, Tlalnepantla de Baz 54090, Mexico., Arias-León J; Faculty of Medicine, Autonomous University of Yucatan, Mérida 97000, Mexico., Machado-Contreras J; Immunology Laboratory, Faculty of Medicine, Autonomous University of Baja California, Mexicali 21000, Mexico., Valencia-Pacheco G; Dr. Hideyo Noguchi Regional Research Center, Autonomous University of Yucatan, Mérida 97000, Mexico., Panti-Balam C; Dr. Hideyo Noguchi Regional Research Center, Autonomous University of Yucatan, Mérida 97000, Mexico., Balam-Romero J; Dr. Hideyo Noguchi Regional Research Center, Autonomous University of Yucatan, Mérida 97000, Mexico., Ku-González A; Yucatan Scientific Research Center, Biochemistry and Plant Molecular Biology Unit, Mérida 97200, Mexico., Peniche-Lara G; Faculty of Medicine, Autonomous University of Yucatan, Mérida 97000, Mexico., Mosqueda J; Immunology and Vaccines Laboratory, College of Natural Sciences, Autonomous University of Queretaro, Queretaro 76010, Mexico., Zazueta OE; Ministry of Health of Baja California, Mexicali 21000, Mexico., Lugo-Caballero C; Dr. Hideyo Noguchi Regional Research Center, Autonomous University of Yucatan, Mérida 97000, Mexico., Puerto-Manzano F; Dr. Hideyo Noguchi Regional Research Center, Autonomous University of Yucatan, Mérida 97000, Mexico. |
| Abstrakt: |
Background: In recent years, promising vaccination strategies against rickettsiosis have been described in experimental animal models and human cells. OmpB is considered an immunodominant antigen that is recognized by T and B cells. The aim of this study was to identify TCD4+INF-γ+ and TCD8+INF-γ+ lymphocytes in an autologous system with macrophages transfected with the vaccine candidate pVAX1-OmpB24. Lymphocytes and monocytes from 14 patients with Rickettsia were isolated from whole blood. Monocytes were differentiated into macrophages and transfected with the plasmid pVAX1-OmpB24 pVax1. Isolated lymphocytes were cultured with transfected macrophages. IFN-γ-producing TCD4+ and TCD8+ lymphocyte subpopulations were identified by flow cytometry, as was the percentage of macrophages expressing CD40+, CD80+, HLA-I and HLA-II. Also, we analyzed the exhausted condition of the T lymphocyte subpopulation by PD1 expression. Macrophages transfected with pVAX1-OmpB24 stimulated TCD4+INF-γ+ cells in healthy subjects and patients infected with R. typhi . Macrophages stimulated TCD8+INF-γ+ cells in healthy subjects and patients infected with R. rickettsii and R. felis . Cells from healthy donors stimulated with OmpB-24 showed a higher percentage of TCD4+PD1+. Cells from patients infected with R. rickettsii had a higher percentage of TCD8+PD-1+, and for those infected with R. typhi the larger number of cells corresponded to TCD4+PD1+. Human macrophages transfected with pVAX1-OmpB24 activated TCD4+IFN-γ+ and CD8+IFN-γ+ in patients infected with different Rickettsia species. However, PD1 expression played an important role in the inhibition of T lymphocytes with R. felis. |
