| Autor: |
Zharkova MS; Institute of Experimental Medicine, WCRC 'Center for Personalized Medicine', 12 Academic Pavlov Street, St. Petersburg 197022, Russia., Komlev AS; Institute of Experimental Medicine, WCRC 'Center for Personalized Medicine', 12 Academic Pavlov Street, St. Petersburg 197022, Russia., Filatenkova TA; Institute of Experimental Medicine, WCRC 'Center for Personalized Medicine', 12 Academic Pavlov Street, St. Petersburg 197022, Russia., Sukhareva MS; Institute of Experimental Medicine, WCRC 'Center for Personalized Medicine', 12 Academic Pavlov Street, St. Petersburg 197022, Russia., Vladimirova EV; Institute of Experimental Medicine, WCRC 'Center for Personalized Medicine', 12 Academic Pavlov Street, St. Petersburg 197022, Russia., Trulioff AS; Institute of Experimental Medicine, WCRC 'Center for Personalized Medicine', 12 Academic Pavlov Street, St. Petersburg 197022, Russia., Orlov DS; Institute of Experimental Medicine, WCRC 'Center for Personalized Medicine', 12 Academic Pavlov Street, St. Petersburg 197022, Russia., Dmitriev AV; Institute of Experimental Medicine, WCRC 'Center for Personalized Medicine', 12 Academic Pavlov Street, St. Petersburg 197022, Russia., Afinogenova AG; St. Petersburg Pasteur Institute, 14 Mira Street, St. Petersburg 197101, Russia., Spiridonova AA; Department of Clinical Microbiology, Pavlov First Saint Petersburg State Medical University, 6/8 Lev Tolstoy Street, St. Petersburg 197022, Russia., Shamova OV; Institute of Experimental Medicine, WCRC 'Center for Personalized Medicine', 12 Academic Pavlov Street, St. Petersburg 197022, Russia.; Department of Biochemistry, Saint Petersburg State University, 7/9 Universitetskaya Embankment, St. Petersburg 199034, Russia. |
| Abstrakt: |
Antimicrobial peptides (AMPs) are acknowledged as a promising template for designing new antimicrobials. At the same time, existing toxicity issues and limitations in their pharmacokinetics make topical application one of the less complicated routes to put AMPs-based therapeutics into actual medical practice. Antiseptics are one of the common components for topical treatment potent against antibiotic-resistant pathogens but often with toxicity limitations of their own. Thus, the interaction of AMPs and antiseptics is an interesting topic that is also less explored than combined action of AMPs and antibiotics. Herein, we analyzed antibacterial, antibiofilm, and cytotoxic activity of combinations of both membranolytic and non-membranolytic AMPs with a number of antiseptic agents. Fractional concentration indices were used as a measure of possible effective concentration reduction achievable due to combined application. Cases of both synergistic and antagonistic interaction with certain antiseptics and surfactants were identified, and trends in the occurrence of these types of interaction were discussed. The data may be of use for AMP-based drug development and suggest that the topic requires further attention for successfully integrating AMPs-based products in the context of complex treatment. AMP/antiseptic combinations show promise for creating topical formulations with improved activity, lowered toxicity, and, presumably, decreased chances of inducing bacterial resistance. However, careful assessment is required to avoid AMP neutralization by certain antiseptic classes in either complex drug design or AMP application alongside other therapeutics/care products. |
