Autor: |
Hashem SM; National Organization for Drug Control and Research, Cairo 12622, Egypt., Gad MK; National Organization for Drug Control and Research, Cairo 12622, Egypt., Anwar HM; Department of Biochemistry, Egyptian Drug Authority, Cairo 12611, Egypt., Saleh NM; Department of Microbiology, Egyptian Drug Authority, Cairo 12611, Egypt., Shamma RN; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt., Elsherif NI; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Heliopolis University, Cairo 11785, Egypt. |
Jazyk: |
angličtina |
Zdroj: |
Pharmaceutics [Pharmaceutics] 2022 Dec 21; Vol. 15 (1). Date of Electronic Publication: 2022 Dec 21. |
DOI: |
10.3390/pharmaceutics15010026 |
Abstrakt: |
Numerous obstacles challenge the treatment of fungal infections, including the uprising resistance and the low penetration of available drugs. One of the main active agents against fungal infections is itraconazole (ITZ), with activity against a broad spectrum of fungi while having few side effects. The aim of this study was to design ufasomes, oleic acid-based colloidal carriers, that could encapsulate ITZ to improve its penetration power. Employing a 2 2 3 1 factorial design, the effect of three independent factors (oleic acid amount, cholesterol concentration, and ITZ amount) was investigated and evaluated for the percentage encapsulation efficiency (%EE), particle size (PS), and zeta potential (ZP). Optimization was performed using Design ® expert software and the optimized ITZ-loaded ufasomes obtained had %EE of 99.4 ± 0.7%, PS of 190 ± 1 nm, and ZP of -81.6 ± 0.4 mV, with spherical unilamellar morphology and no aggregation. An in vitro microbiological study was conducted to identify the minimum inhibitory concentration of the selected formula against Candida albicans , which was found to be 0.0625 μg/mL. Moreover, the optimized formula reduced the expression of toll-like receptors-4 and pro-inflammatory cytokine IL-1β secretion in the C. albicans -infected fibroblasts, indicating that the proposed ITZ-loaded ufasomes are a promising drug delivery system for ITZ. |
Databáze: |
MEDLINE |
Externí odkaz: |
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