| Autor: |
López-Rodríguez G; Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km 1. Ex-Hda. de Aquetzalpa, Tulancingo C.P. 43600, Hidalgo, Mexico., Zaragoza-Bastida A; Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km 1. Ex-Hda. de Aquetzalpa, Tulancingo C.P. 43600, Hidalgo, Mexico., Reyes-Guerrero DE; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID SAI-INIFAP), Carrtera Federal Cuernavaca-Cuautla No. 8534/Col. Progreso, Jiutepec C.P. 62550, Morelos, Mexico., Olmedo-Juárez A; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID SAI-INIFAP), Carrtera Federal Cuernavaca-Cuautla No. 8534/Col. Progreso, Jiutepec C.P. 62550, Morelos, Mexico., Valladares-Carranza B; Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, km 15.5 Carretera Panamericana, Toluca-Atlacomulco, Toluca C.P. 50200, Estado de México, Mexico., Vega-Castillo LF; Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, km 15.5 Carretera Panamericana, Toluca-Atlacomulco, Toluca C.P. 50200, Estado de México, Mexico., Rivero-Perez N; Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km 1. Ex-Hda. de Aquetzalpa, Tulancingo C.P. 43600, Hidalgo, Mexico. |
| Abstrakt: |
Goat production in Mexico is an important economic activity that is affected by different gastrointestinal nematode (GIN) species. GINs resistant to commercial anthelmintics have been reported. Plant extracts or agro-industrial by-products, such as coffee pulp, have been proposed as control alternatives, given their secondary metabolite content. The aim of the present study was to determine the anthelmintic activity of the hydroalcoholic extract of coffee pulp against benzimidazole-resistant GINs. Stool samples were collected from goats, from which GIN eggs were identified and quantified. Molecular techniques confirmed the genus of GINs and their benzimidazole resistance profile. The percentage of egg hatching inhibition (% EHI) and larval mortality (% LM) with the hydroalcoholic extract of coffee pulp was determined at concentrations from 200 to 0.39 mg/mL. The genera Haemonchus spp. and Trichostrongylus spp. were identified, and the presence of the β-tubulin gene mutation, associated with benzimidazole (BZ) resistance, was determined. Hydroalcoholic extract of coffee pulp inhibited 100% of egg hatching at 200 and 100 mg/mL, with no larvicidal effect at the evaluated concentrations. |
