Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38.

Autor: Maloney JG; Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA., Molokin A; Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA., Seguí R; Department of Pharmacy, Pharmaceutical Technology and Parasitology, Parasitology Area, Faculty of Pharmacy, Valencia University, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.; Global Omnium, Gran Via del Marqués del Túria 19, 46005 Valencia, Valencia, Spain., Maravilla P; Departamento de Ecologia de Agentes Patogenos, Hospital General Dr. Manuel Gea Gonzalez, Mexico City 14080, Mexico., Martínez-Hernández F; Departamento de Ecologia de Agentes Patogenos, Hospital General Dr. Manuel Gea Gonzalez, Mexico City 14080, Mexico., Villalobos G; Departamento de Ecologia de Agentes Patogenos, Hospital General Dr. Manuel Gea Gonzalez, Mexico City 14080, Mexico., Tsaousis AD; Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK., Gentekaki E; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand., Muñoz-Antolí C; Department of Pharmacy, Pharmaceutical Technology and Parasitology, Parasitology Area, Faculty of Pharmacy, Valencia University, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain., Klisiowicz DR; Department of Basic Pathology, Biological Sciences Area, Paraná Federal University, Av. Cel. Francisco H. dos Santos 100, Curitiba 19031, Brazil., Oishi CY; Department of Basic Pathology, Biological Sciences Area, Paraná Federal University, Av. Cel. Francisco H. dos Santos 100, Curitiba 19031, Brazil., Toledo R; Department of Pharmacy, Pharmaceutical Technology and Parasitology, Parasitology Area, Faculty of Pharmacy, Valencia University, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain., Esteban JG; Department of Pharmacy, Pharmaceutical Technology and Parasitology, Parasitology Area, Faculty of Pharmacy, Valencia University, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain., Köster PC; Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28222 Madrid, Spain., de Lucio A; Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28222 Madrid, Spain., Dashti A; Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28222 Madrid, Spain., Bailo B; Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28222 Madrid, Spain., Calero-Bernal R; SALUVET, Department of Animal Health, Faculty of Veterinary, Complutense University of Madrid, 28040 Madrid, Spain., González-Barrio D; Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28222 Madrid, Spain., Carmena D; Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28222 Madrid, Spain.; Center for Biomedical Research Network in Infectious Diseases (CIBERINFEC), Health Institute Carlos III, 28222 Madrid, Spain., Santín M; Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA.
Jazyk: angličtina
Zdroj: Microorganisms [Microorganisms] 2022 Dec 23; Vol. 11 (1). Date of Electronic Publication: 2022 Dec 23.
DOI: 10.3390/microorganisms11010046
Abstrakt: Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were performed to confirm the validity of each of these new subtypes. We propose that the novel sequences described in this study should be assigned the subtype designations ST35-ST38. The full-length reference sequences of ST35-ST38 will assist in accurate sequence descriptions in future studies of Blastocystis epidemiology and subtype diversity.
Databáze: MEDLINE