"Silicon-On-Insulator"-Based Biosensor for the Detection of MicroRNA Markers of Ovarian Cancer.

Autor: Ivanov YD; Institute of Biomedical Chemistry (IBMC), 119121 Moscow, Russia.; Joint Institute for High Temperatures of Russian Academy of Sciences, 125412 Moscow, Russia., Kapustina SI; Institute of Biomedical Chemistry (IBMC), 119121 Moscow, Russia.; Department of Cybernetics of Chemical and Technological Processes, Mendeleev University of Chemical Technology of Russia (MUCTR), 125047 Moscow, Russia., Malsagova KA; Institute of Biomedical Chemistry (IBMC), 119121 Moscow, Russia., Goldaeva KV; Institute of Biomedical Chemistry (IBMC), 119121 Moscow, Russia., Pleshakova TO; Institute of Biomedical Chemistry (IBMC), 119121 Moscow, Russia., Galiullin RA; Institute of Biomedical Chemistry (IBMC), 119121 Moscow, Russia., Shumov ID; Institute of Biomedical Chemistry (IBMC), 119121 Moscow, Russia., Kozlov AF; Institute of Biomedical Chemistry (IBMC), 119121 Moscow, Russia., Glukhov AV; JSC 'Novosibirsk Plant of Semiconductor Devices with OKB', 630082 Novosibirsk, Russia., Grabezhova VK; JSC 'Design Center for Biomicroelectronic Technologies 'Vega'', 630082 Novosibirsk, Russia., Popov VP; Rzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia., Petrov OF; Joint Institute for High Temperatures of Russian Academy of Sciences, 125412 Moscow, Russia., Ziborov VS; Institute of Biomedical Chemistry (IBMC), 119121 Moscow, Russia.; Joint Institute for High Temperatures of Russian Academy of Sciences, 125412 Moscow, Russia., Kushlinskii NE; N.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, Russia., Alferov AA; N.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, Russia., Konev VA; Department of Infectious Diseases in Children, Faculty of Pediatrics, Pirogov Russian National Research Medical University (RNRMU), 117997 Moscow, Russia., Kovalev OB; Department of Infectious Diseases in Children, Faculty of Pediatrics, Pirogov Russian National Research Medical University (RNRMU), 117997 Moscow, Russia., Uchaikin VF; Department of Infectious Diseases in Children, Faculty of Pediatrics, Pirogov Russian National Research Medical University (RNRMU), 117997 Moscow, Russia., Archakov AI; Institute of Biomedical Chemistry (IBMC), 119121 Moscow, Russia.
Jazyk: angličtina
Zdroj: Micromachines [Micromachines (Basel)] 2022 Dec 27; Vol. 14 (1). Date of Electronic Publication: 2022 Dec 27.
DOI: 10.3390/mi14010070
Abstrakt: Ovarian cancer is a gynecological cancer characterized by a high mortality rate and tumor heterogeneity. Its early detection and primary prophylaxis are difficult to perform. Detecting biomarkers for ovarian cancer plays a pivotal role in therapy effectiveness and affects patients' survival. This study demonstrates the detection of microRNAs (miRNAs), which were reported to be associated with ovarian cancer tumorigenesis, with a nanowire biosensor based on silicon-on-insulator structures (SOI-NW biosensor). The advantages of the method proposed for miRNA detection using the SOI-NW biosensor are as follows: (1) no need for additional labeling or amplification reaction during sample preparation, and (2) real-time detection of target biomolecules. The detecting component of the biosensor is a chip with an array of 3 µm wide, 10 µm long silicon nanowires on its surface. The SOI-NW chip was fabricated using the "top-down" method, which is compatible with large-scale CMOS technology. Oligonucleotide probes (oDNA probes) carrying sequences complementary to the target miRNAs were covalently immobilized on the nanowire surface to ensure high-sensitivity biospecific sensing of the target biomolecules. The study involved two experimental series. Detection of model DNA oligonucleotides being synthetic analogs of the target miRNAs was carried out to assess the method's sensitivity. The lowest concentration of the target oligonucleotides detectable in buffer solution was 1.1 × 10 -16 M. In the second experimental series, detection of miRNAs (miRNA-21, miRNA-141, and miRNA-200a) isolated from blood plasma samples collected from patients having a verified diagnosis of ovarian cancer was performed. The results of our present study represent a step towards the development of novel highly sensitive diagnostic systems for the early revelation of ovarian cancer in women.
Databáze: MEDLINE
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