Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes.

Autor:	Slotvitsky M; Moscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, Russia.; M.F. Vladimirsky Moscow Regional Clinical Research Institute, Schepkina St. 61/2, 129110 Moscow, Russia., Berezhnoy A; Moscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, Russia.; M.F. Vladimirsky Moscow Regional Clinical Research Institute, Schepkina St. 61/2, 129110 Moscow, Russia., Scherbina S; Moscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, Russia., Rimskaya B; Moscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, Russia., Tsvelaya V; Moscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, Russia.; M.F. Vladimirsky Moscow Regional Clinical Research Institute, Schepkina St. 61/2, 129110 Moscow, Russia., Balashov V; Moscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, Russia., Efimov AE; Academician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya St., 1, 123182 Moscow, Russia., Agapov I; Academician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya St., 1, 123182 Moscow, Russia., Agladze K; Moscow Institute of Physics and Technology, Institutskiy Lane 9, 141700 Dolgoprudny, Russia.; M.F. Vladimirsky Moscow Regional Clinical Research Institute, Schepkina St. 61/2, 129110 Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Micromachines [Micromachines (Basel)] 2022 Dec 25; Vol. 14 (1). Date of Electronic Publication: 2022 Dec 25.
DOI:	10.3390/mi14010051
Abstrakt:	Induced pluripotent stem cells (iPSCs) constitute a potential source of patient-specific human cardiomyocytes for a cardiac cell replacement therapy via intramyocardial injections, providing a major benefit over other cell sources in terms of immune rejection. However, intramyocardial injection of the cardiomyocytes has substantial challenges related to cell survival and electrophysiological coupling with recipient tissue. Current methods of manipulating cell suspensions do not allow one to control the processes of adhesion of injected cells to the tissue and electrophysiological coupling with surrounding cells. In this article, we documented the possibility of influencing these processes using polymer kernels: biocompatible fiber fragments of subcellular size that can be adsorbed to a cell, thereby creating the minimum necessary adhesion foci to shape the cell and provide support for the organization of the cytoskeleton and the contractile apparatus prior to adhesion to the recipient tissue. Using optical excitation markers, the restoration of the excitability of cardiomyocytes in suspension upon adsorption of polymer kernels was shown. It increased the likelihood of the formation of a stable electrophysiological coupling in vitro. The obtained results may be considered as a proof of concept that the stochastic engraftment process of injected suspension cells can be controlled by smart biomaterials.
Databáze:	MEDLINE
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