| Autor: |
Dalamu; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India., Tiwari JK; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India., Bairwa A; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India., Bhatia N; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India.; School of Biotechnology, Shoolini University, Solan 173229, Himachal Pradesh, India., Zinta R; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India.; School of Bioengineering and Biosciences, Lovely Professional University, Phagwara 144001, Punjab, India., Kaushal N; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India., Kumar V; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India., Sharma AK; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India., Sharma S; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India., Choudhary B; ICAR-Central Potato Research Institute, Regional Station, Modipuram, Meerut 250110, Uttar Pradesh, India., Luthra SK; ICAR-Central Potato Research Institute, Regional Station, Modipuram, Meerut 250110, Uttar Pradesh, India., Buckseth T; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India., Singh RK; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India., Thakur AK; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India., Kumar M; ICAR-Central Potato Research Institute, Regional Station, Modipuram, Meerut 250110, Uttar Pradesh, India., Kumar D; Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India.; ICAR-Central Potato Research Institute, Regional Station, Modipuram, Meerut 250110, Uttar Pradesh, India. |
| Abstrakt: |
The potato originated in southern Peru and north-western Bolivia (South America). However, native accessions have also been cultivated in India for many years. Late blight, caused by the fungus Phytophthora infestans , is the most devastating potato disease, while potato cyst nematode ( Globodera spp.) (PCN) is another economically significant quarantine-requiring pest in India. In this study, we have generated a new Indian native collection of 94 potato accessions collected from different parts India. These accessions were screened against late blight and potato cyst nematode resistance by using gene-based molecular markers and phenotypic screening methods. Marker assisted selection using R1 gene-specific marker CosA 210 revealed a late blight resistance gene in 11 accessions. PCN resistance bands were found in 3 accessions with marker TG689 141 , 5 accessions with marker 57R 452 , and 1 accession having Gro1-4-1 602 marker for G. rostochiensis (Ro1,4), while 64 accessions amplified marker HC 276 indicating G. pallida (Pa2,3) resistance gene ( GpaV vrn QTL ). On the other hand, phenotypic screening against late blight resistance under natural epiphytic conditions (hot-spot) revealed three accessions with high resistance, while others were resistant (1 accession), moderately resistant (5 accessions), susceptible (29 accessions), and highly susceptible (56 accessions). For G. rostochiensis (golden cyst nematode) and G. pallida (white cyst nematode) resistance, accessions were grouped into highly resistant (3, 3), resistant (0, 2), moderately resistant (6, 29), susceptible (32, 30), and highly susceptible (53, 30), respectively, for the two PCN species. Collectively, we identified promising accessions with high resistance to late blight (JG-1, Kanpuria Safed, and Rangpuria), and also highly resistant to both Globodera species (Garlentic, Jeevan Jyoti, and JG-1). Our findings suggested that these accessions would be useful for late blight and PCN resistance breeding, as well as future molecular studies in potatoes. |
