Bactericidal Properties of Proline-Rich Aedes aegypti Trypsin Modulating Oostatic Factor ( Aea TMOF).

Autor: Borovsky D; Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA., Rougé P; Faculte des Sciences Pharmaceutiques, 3106 Toulouse, France., Shatters RG Jr; USDA-ARS, Horticultural Research Laboratory, Ft. Pierce, FL 34945, USA.
Jazyk: angličtina
Zdroj: Life (Basel, Switzerland) [Life (Basel)] 2022 Dec 21; Vol. 13 (1). Date of Electronic Publication: 2022 Dec 21.
DOI: 10.3390/life13010019
Abstrakt: The antimicrobial properties of proline-rich Aedes aegypti decapeptide TMOF ( Aea TMOF) and oncocin112 (1-13) were compared. Incubations with multidrug-resistant Escherichia coli cells showed that Aea TMOF (5 mM) was able to completely inhibit bacterial cell growth, whereas oncocin112 (1-13) (20 mM) partially inhibited bacterial growth as compared with bacterial cells that were not multidrug-resistant cells. Aea TMOF (5 mM) was very effective against Acinetobacter baumannii and Pseudomonas aeruginosa , completely inhibiting cell growth during 15 h incubations. Aea TMOF (5 mM) completely inhibited the Gram-positive bacteria Staphylococcus aureus and Bacillus thurengiensis sups. Israelensis cell growth, whereas oncocin112 (1-13) (10 and 20 mM) failed to affect bacterial cell growth. E. coli cells that lack the SbmA transporter were inhibited by Aea TMOF (5 mM) and not by oncocin112 (1-13) (10 to 20 mM), indicating that Aea TMOF can use other bacterial transporters than SbmA that is mainly used by proline-rich antimicrobial peptides. Incubation of E. coli cells with NaAzide showed that Aea TMOF does not use ABC-like transporters that use ATP hydrolysis to import molecules into bacterial cells. Three-dimensional modeling and docking of AeaTMOF to SbmA and MdtM transporters showed that Aea TMOF can bind these proteins, and the binding location of AeaTMOF inside these protein transporters allows Aea TMOF to be transported into the bacterial cytosol. These results show that Aea TMOF can be used as a future antibacterial agent against both multidrug-resistant Gram-positive and -negative bacteria.
Databáze: MEDLINE
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