| Autor: |
Azevedo MJ; INEB-Instituto Nacional de Engenharia Biomédica, 4150-177 Porto, Portugal.; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal.; Department of Preventive Dentistry, Academic Center for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The Netherlands., Araujo R; INEB-Instituto Nacional de Engenharia Biomédica, 4150-177 Porto, Portugal.; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal., Campos J; INEB-Instituto Nacional de Engenharia Biomédica, 4150-177 Porto, Portugal.; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal., Campos C; Serviço de Patologia Clínica, Departamento de Patologia e Medicina Laboratorial, Instituto Português de Oncologia do Porto Francisco Gentil, 4200-072 Porto, Portugal.; Escola Superior de Saúde, Instituto Politécnico do Porto, 4200-072 Porto, Portugal., Ferreira AF; Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal., Falcão-Pires I; Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal., Ramalho C; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal.; Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal.; Department of Gynecology and Obstetrics, Centro Hospitalar Universitário de São João, 4200-319 Porto, Portugal., Zaura E; Department of Preventive Dentistry, Academic Center for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The Netherlands., Pinto E; Faculdade de Farmácia, Universidade do Porto, 4050-313 Porto, Portugal.; Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Universidade do Porto, 4450-208 Matosinhos, Portugal., Sampaio-Maia B; INEB-Instituto Nacional de Engenharia Biomédica, 4150-177 Porto, Portugal.; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal.; Faculdade de Medicina Dentária, Universidade do Porto, 4200-393 Porto, Portugal. |
| Abstrakt: |
Yeast acquisition begins at birth; however, the contribution of the mother on yeast transmission to the offspring and associated resistance is yet to be clarified. The aim of this study was to explore the vertical transmission of yeasts and their antifungal susceptibility profile in early life. Oral, fecal, and breastmilk samples were collected from 73 mother-child pairs four to twelve weeks after delivery and cultured on Sabouraud dextrose agar with chloramphenicol. The isolates were identified by MALDI-TOF MS. The vertical transmission was studied by microsatellite genotyping. Antifungal susceptibility was determined for fluconazole, voriconazole, miconazole, anidulafungin, and nystatin by broth microdilution assay, following CLSI-M60 guidelines. A total of 129 isolates were identified from 53% mother-child pairs. We verified the vertical transmission of Candida albicans (n = three mother-child pairs) and Candida parapsilosis (n = one mother-child pair) strains, including an antifungal resistant strain transmitted from breastmilk to the gut of a child. Most isolates were susceptible to the tested antifungals, with the exception of four C. albicans isolates and one R. mucilaginosa isolate. The vertical transmission of yeasts happens in early life. This is the first work that demonstrated the role of the mother as a source of transmission of antifungal-resistant yeasts to the child. |
