| Autor: |
Ceccarini MR; Department of Pharmaceutical Sciences, University of Perugia, 06123 Perugia, Italy., Palazzi V; Department of Engineering, University of Perugia, 06125 Perugia, Italy., Salvati R; Department of Engineering, University of Perugia, 06125 Perugia, Italy., Chiesa I; Department of Ingegneria dell'Informazione and Research Center E. Piaggio, University of Pisa, Largo Lucio Lazzarino 1, 56122 Pisa, Italy., De Maria C; Department of Ingegneria dell'Informazione and Research Center E. Piaggio, University of Pisa, Largo Lucio Lazzarino 1, 56122 Pisa, Italy., Bonafoni S; Department of Engineering, University of Perugia, 06125 Perugia, Italy., Mezzanotte P; Department of Engineering, University of Perugia, 06125 Perugia, Italy., Codini M; Department of Pharmaceutical Sciences, University of Perugia, 06123 Perugia, Italy., Pacini L; Interdepartmental Research Unit of Peptide and Protein Chemistry and Biology, Department of Chemistry 'Ugo Schiff', University of Florence, 50019 Sesto Fiorentino, Italy., Errante F; Interdepartmental Research Unit of Peptide and Protein Chemistry and Biology, Department of NEUROFARBA, University of Florence, 50019 Sesto Fiorentino, Italy., Rovero P; Interdepartmental Research Unit of Peptide and Protein Chemistry and Biology, Department of NEUROFARBA, University of Florence, 50019 Sesto Fiorentino, Italy., Morabito A; Dipartimento Neuroscienze, Psicologia, Area del Farmaco e Salute del Bambino NEUROFARBA, Università degli Studi di Firenze, Viale Pieraccini 6, 50121 Firenze, Italy., Beccari T; Department of Pharmaceutical Sciences, University of Perugia, 06123 Perugia, Italy., Roselli L; Department of Engineering, University of Perugia, 06125 Perugia, Italy., Valentini L; Civil and Environmental Engineering Department, University of Perugia, Strada di Pentima 4, 05100 Terni, Italy. |
| Abstrakt: |
This study illustrates the sensing and wound healing properties of silk fibroin in combination with peptide patterns, with an emphasis on the printability of multilayered grids, and envisions possible applications of these next-generation silk-based materials. Functionalized silk fibers covalently linked to an arginine-glycine-aspartic acid (RGD) peptide create a platform for preparing a biomaterial ink for 3D printing of grid-like piezoresistors with wound-healing and sensing properties. The culture medium obtained from 3D-printed silk fibroin enriched with RGD peptide improves cell adhesion, accelerating skin repair. Specifically, RGD peptide-modified silk fibroin demonstrated biocompatibility, enhanced cell adhesion, and higher wound closure rates at lower concentration than the neat peptide. It was also shown that the printing of peptide-modified silk fibroin produces a piezoresistive transducer that is the active component of a sensor based on a Schottky diode harmonic transponder encoding information about pressure. We discovered that such biomaterial ink printed in a multilayered grid can be used as a humidity sensor. Furthermore, humidity activates a transition between low and high conductivity states in this medium that is retained unless a negative voltage is applied, paving the way for utilization in non-volatile organic memory devices. Globally, these results pave the way for promising applications, such as monitoring parameters such as human wound care and being integrated in bio-implantable processors. |
