F 4 -Neuroprostane Effects on Human Sperm.

Autor: Moretti E; Department of Molecular and Developmental Medicine, Policlinico Le Scotte, University of Siena, 53100 Siena, Italy., Signorini C; Department of Molecular and Developmental Medicine, Policlinico Le Scotte, University of Siena, 53100 Siena, Italy., Noto D; Department of Molecular and Developmental Medicine, Policlinico Le Scotte, University of Siena, 53100 Siena, Italy., Corsaro R; Department of Molecular and Developmental Medicine, Policlinico Le Scotte, University of Siena, 53100 Siena, Italy., Micheli L; Department of Medicine, Surgery and Neurosciences, University of Siena, 53100 Siena, Italy., Durand T; Institut des Biomolécules Max Mousseron (IBMM), Pole Chimie Balard Recherche, UMR 5247, CNRS, Université de Montpellier, ENSCM, 34090 Montpellier, France., Oger C; Institut des Biomolécules Max Mousseron (IBMM), Pole Chimie Balard Recherche, UMR 5247, CNRS, Université de Montpellier, ENSCM, 34090 Montpellier, France., Galano JM; Institut des Biomolécules Max Mousseron (IBMM), Pole Chimie Balard Recherche, UMR 5247, CNRS, Université de Montpellier, ENSCM, 34090 Montpellier, France., Collodel G; Department of Molecular and Developmental Medicine, Policlinico Le Scotte, University of Siena, 53100 Siena, Italy.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 Jan 04; Vol. 24 (2). Date of Electronic Publication: 2023 Jan 04.
DOI: 10.3390/ijms24020935
Abstrakt: Swim-up selected human sperm were incubated with 7 ng F 4 -neuroprostanes (F 4 -NeuroPs) for 2 and 4 h. Sperm motility and membrane mitochondrial potential (MMP) were evaluated. The percentage of reacted acrosome was assessed by pisum sativum agglutinin (PSA). Chromatin integrity was detected using the acridine orange (AO) assay and localization of the ryanodine receptor was performed by immunofluorescence analysis. Sperm progressive motility ( p = 0.02) and the percentage of sperm showing a strong MMP signal ( p = 0.012) significantly increased after 2 h F 4 -NeuroP incubation compared to control samples. The AO assay did not show differences in the percentage of sperm with dsDNA between treated or control samples. Meanwhile, a significantly higher number of sperm with reacted acrosomes was highlighted by PSA localization after 4 h F 4 -NeuroP incubation. Finally, using an anti-ryanodine antibody, the immunofluorescence signal was differentially distributed at 2 and 4 h: a strong signal was evident in the midpiece and postacrosomal sheath (70% of sperm) at 2 h, whereas a dotted one appeared at 4 h (53% of sperm). A defined concentration of F 4 -NeuroPs in seminal fluid may induce sperm capacitation via channel ions present in sperm cells, representing an aid during in vitro sperm preparation that may increase the positive outcome of assisted fertilization.
Databáze: MEDLINE
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