| Autor: |
Mastromarino MG; Division of Thoracic Surgery, Ospedale Maggiore della Carità di Novara, 28100 Novara, Italy., Parini S; Division of Thoracic Surgery, Ospedale Maggiore della Carità di Novara, 28100 Novara, Italy., Azzolina D; Department of Medical Sciences, University of Ferrara, 44121 Ferrara, Italy., Habib S; Division of Thoracic Surgery, Ospedale Maggiore della Carità di Novara, 28100 Novara, Italy., De Marni ML; Tethis S.p.A., 20143 Milan, Italy., Luise C; Tethis S.p.A., 20143 Milan, Italy., Restelli S; Tethis S.p.A., 20143 Milan, Italy., Baietto G; Division of Thoracic Surgery, Ospedale Maggiore della Carità di Novara, 28100 Novara, Italy., Trisolini E; Division of Pathology, Ospedale Maggiore della Carità di Novara, 28100 Novara, Italy., Massera F; Division of Thoracic Surgery, Ospedale Maggiore della Carità di Novara, 28100 Novara, Italy., Papalia E; Division of Thoracic Surgery, Ospedale Maggiore della Carità di Novara, 28100 Novara, Italy., Bora G; Division of Thoracic Surgery, Ospedale Maggiore della Carità di Novara, 28100 Novara, Italy., Carbone R; Tethis S.p.A., 20143 Milan, Italy., Casadio C; Division of Thoracic Surgery, Ospedale Maggiore della Carità di Novara, 28100 Novara, Italy., Boldorini R; Division of Pathology, Ospedale Maggiore della Carità di Novara, 28100 Novara, Italy.; Department of Health Sciences, University of Piemonte Orientale, 28100 Novara, Italy., Rena O; Division of Thoracic Surgery, Ospedale Maggiore della Carità di Novara, 28100 Novara, Italy.; Department of Health Sciences, University of Piemonte Orientale, 28100 Novara, Italy. |
| Abstrakt: |
Lung cancer is still the leading cause of cancer-related death worldwide. Interest is growing towards early detection and advances in liquid biopsy to isolate circulating tumor cells (CTCs). This pilot study aimed to detect epithelial CTCs in the peripheral blood of early-stage non-small cell lung cancer (NSCLC) patients. We used Smart BioSurface ® (SBS) slide, a nanoparticle-coated slide able to immobilize viable nucleated cellular fraction without pre-selection and preserve cell integrity. Forty patients undergoing lung resection for NSCLC were included; they were divided into two groups according to CTC value, with a cut-off of three CTCs/mL. All patients were positive for CTCs. The mean CTC value was 4.7(± 5.8 S.D.) per ml/blood. In one patient, next generation sequencing (NGS) analysis of CTCs revealed v-raf murine sarcoma viral oncogene homolog B(BRAF) V600E mutation, which has also been identified in tissue biopsy. CTCs count affected neither overall survival (OS, p = 0.74) nor progression-free survival ( p = 0.829). Multivariable analysis confirmed age ( p = 0.020) and pNodal-stage ( p = 0.028) as negative predictors of OS. Preliminary results of this pilot study suggest the capability of this method in detecting CTCs in all early-stage NSCLC patients. NGS on single cell, identified as CTC by immunofluorescence staining, is a powerful tool for investigating the molecular landscape of cancer, with the aim of personalized therapies. |
