| Autor: |
Balestri W; Department of Engineering, School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, UK., Hickman GJ; Imaging Suite, School of Science & Technology, Nottingham Trent University, Nottingham NG11 8NS, UK., Morris RH; Department of Physics and Mathematics, School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, UK., Hunt JA; Medical Technologies and Advanced Materials, School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, UK.; College of Biomedical Engineering, China Medical University, Taichung 40402, Taiwan., Reinwald Y; Department of Engineering, School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, UK. |
| Jazyk: |
angličtina |
| Zdroj: |
Cells [Cells] 2023 Jan 13; Vol. 12 (2). Date of Electronic Publication: 2023 Jan 13. |
| DOI: |
10.3390/cells12020313 |
| Abstrakt: |
The transition areas between different tissues, known as tissue interfaces, have limited ability to regenerate after damage, which can lead to incomplete healing. Previous studies focussed on single interfaces, most commonly bone-tendon and bone-cartilage interfaces. Herein, we develop a 3D in vitro model to study the regeneration of the bone-tendon-muscle interface. The 3D model was prepared from collagen and agarose, with different concentrations of hydroxyapatite to graduate the tissues from bones to muscles, resulting in a stiffness gradient. This graduated structure was fabricated using indirect 3D printing to provide biologically relevant surface topographies. MG-63, human dermal fibroblasts, and Sket.4U cells were found suitable cell models for bones, tendons, and muscles, respectively. The biphasic and triphasic hydrogels composing the 3D model were shown to be suitable for cell growth. Cells were co-cultured on the 3D model for over 21 days before assessing cell proliferation, metabolic activity, viability, cytotoxicity, tissue-specific markers, and matrix deposition to determine interface formations. The studies were conducted in a newly developed growth chamber that allowed cell communication while the cell culture media was compartmentalised. The 3D model promoted cell viability, tissue-specific marker expression, and new matrix deposition over 21 days, thereby showing promise for the development of new interfaces. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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