| Autor: |
Novoyatlova US; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia., Kessenikh AG; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia.; Laboratory for Microbiology, BIOTECH University, 125080 Moscow, Russia., Kononchuk OV; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia.; Laboratory for Microbiology, BIOTECH University, 125080 Moscow, Russia., Bazhenov SV; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia.; Faculty of Physics, HSE University, 109028 Moscow, Russia., Fomkin AA; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia., Kudryavtseva AA; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia.; Faculty of Physics, HSE University, 109028 Moscow, Russia.; Federal Research Center of Biological Systems and Agro-Technologies of RAS, 460000 Orenburg, Russia., Shorunov SV; Topchiev Institute of Petrochemical Synthesis, RAS, 119071 Moscow, Russia., Bermeshev MV; Topchiev Institute of Petrochemical Synthesis, RAS, 119071 Moscow, Russia., Manukhov IV; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia. |
| Abstrakt: |
Dicyclopropanated 5-vinyl-2-norbornene (dcpVNB) is a strained polycyclic hydrocarbon compound with a high energy content, which makes it promising for the development of propellant components based on it. In this work, the genotoxic properties of dcpVNB were studied using whole-cell lux -biosensors based on Escherichia coli and Bacillus subtilis . It was shown that the addition of dcpVNB to bacterial cells leads to the appearance of DNA damage inducing the SOS response and Dps expression with slight activation of the OxyR-mediated response to oxidative stress. The highest toxic effect of dcpVNB is detected by the following lux -biosensors: E. coli pColD-lux, E. coli pDps, B. subtilis pNK-DinC, and B. subtilis pNK-MrgA, in which the genes of bacterial luciferases are transcriptionally fused to the corresponding promoters: P cda , P dps , P dinC , and P mrgA . It was shown that lux -biosensors based on B. subtilis, and E. coli are almost equally sensitive to dcpVNB, which indicates the same permeability to this compound of cell wall of Gram-positive and Gram-negative bacteria. The activation of P dps after dcpVNB addition maintains even in oxyR mutant E. coli strains, which means that the P dps induction is only partially determined by the OxyR/S regulon. Comparison of specific stress effects caused by dcpVNB and 2-ethyl(bicyclo[2.2.1]heptane) (EBH), characterized by the absence of cyclopropanated groups, shows that structural changes in hydrocarbons could significantly change the mode of toxicity. |
