| Autor: |
Hossain MMK; Animal Biotechnology Division, National Institute of Biotechnology, Savar 1349, Bangladesh., Islam MS; Animal Biotechnology Division, National Institute of Biotechnology, Savar 1349, Bangladesh.; Center for Cancer Immunology, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China., Uddin MS; Animal Biotechnology Division, National Institute of Biotechnology, Savar 1349, Bangladesh.; Department of Applied Nutrition and Food Technology, Islamic University, Kushtia 7003, Bangladesh., Rahman ATMM; Department of Applied Nutrition and Food Technology, Islamic University, Kushtia 7003, Bangladesh., Ud-Daula A; Department of Applied Nutrition and Food Technology, Islamic University, Kushtia 7003, Bangladesh., Islam MA; Animal Biotechnology Division, National Institute of Biotechnology, Savar 1349, Bangladesh.; Department of Applied Nutrition and Food Technology, Islamic University, Kushtia 7003, Bangladesh., Rubaya R; Animal Biotechnology Division, National Institute of Biotechnology, Savar 1349, Bangladesh., Bhuiya AA; Animal Biotechnology Division, National Institute of Biotechnology, Savar 1349, Bangladesh., Alim MA; Animal Biotechnology Division, National Institute of Biotechnology, Savar 1349, Bangladesh., Jahan N; Animal Biotechnology Division, National Institute of Biotechnology, Savar 1349, Bangladesh., Li J; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China., Alam J; Animal Biotechnology Division, National Institute of Biotechnology, Savar 1349, Bangladesh. |
| Abstrakt: |
Antimicrobials have been used to improve animal welfare, food security, and food safety that promote the emergence, selection, and dissemination of antimicrobial-resistant (AMR) bacteria. In this study, 50 E. coli were isolated from frozen chicken meat samples in Dhaka city. Antibiotic sensitivity patterns were assessed through the disk diffusion method and finally screened for the presence of antimicrobial resistance genes (ARG) using the polymerase chain reaction (PCR). Among the 160 samples, the prevalence of E. coli was observed in fifty samples (31.25%). All of these isolates were found resistant to at least one antimicrobial agent, and 52.0% of the isolates were resistant against 4-7 different antimicrobials. High resistance was shown to tetracycline (66.0%), followed by resistance to erythromycin (42.0%), ampicillin and streptomycin (38.0%), and sulfonamide (28.0%). In addition, the most prevalent ARGs were tet(A) (66.0%), ereA (64.0%), tet(B) (60.0%), aadA1 and sulI (56.0%), blaCITM (48.0%) and blaSHV (40.0%). About 90.0% of isolates were multidrug resistant. This study reveals for the first time the current situation of E. coli AMR in broilers, which is helpful for the clinical control of disease as well as for the development of policies and guidelines to reduce AMR in broilers production in Bangladesh. |
