3D-printed sensor decorated with nanomaterials by CO 2 laser ablation and electrochemical treatment for non-enzymatic tyrosine detection.

Autor: Veloso WB; Institute of Chemistry, Department of Fundamental Chemistry, University of São Paulo, São Paulo, SP, 05508-000, Brazil., Ataide VN; Institute of Chemistry, Department of Fundamental Chemistry, University of São Paulo, São Paulo, SP, 05508-000, Brazil., Rocha DP; Federal Institute of Paraná, Pitanga, PR, 85200-000, Brazil., Nogueira HP; Institute of Chemistry, Department of Fundamental Chemistry, University of São Paulo, São Paulo, SP, 05508-000, Brazil.; Department of Physical Chemistry, Institute of Chemistry, University of Campinas, Campinas, SP, 13083-970, Brazil., de Siervo A; Institute of Physics 'Gleb Wataghin,' Applied Physics Department, State University of Campinas, Campinas, SP, 13083-859, Brazil., Angnes L; Institute of Chemistry, Department of Fundamental Chemistry, University of São Paulo, São Paulo, SP, 05508-000, Brazil., Muñoz RAA; Institute of Chemistry, Federal University of Uberlândia, Uberlândia, MG, 38400-902, Brazil., Paixão TRLC; Institute of Chemistry, Department of Fundamental Chemistry, University of São Paulo, São Paulo, SP, 05508-000, Brazil. trlcp@iq.usp.br.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2023 Jan 21; Vol. 190 (2), pp. 63. Date of Electronic Publication: 2023 Jan 21.
DOI: 10.1007/s00604-023-05648-8
Abstrakt: The combination of CO 2 laser ablation and electrochemical surface treatments is demonstrated to improve the electrochemical performance of carbon black/polylactic acid (CB/PLA) 3D-printed electrodes through the growth of flower-like Na 2 O nanostructures on their surface. Scanning electron microscopy images revealed that the combination of treatments ablated the electrode's polymeric layer, exposing a porous surface where Na 2 O flower-like nanostructures were formed. The electrochemical performance of the fabricated electrodes was measured by the reversibility of the ferri/ferrocyanide redox couple presenting a significantly improved performance compared with electrodes treated by only one of the steps. Electrodes treated by the combined method also showed a better electrochemical response for tyrosine oxidation. These electrodes were used as a non-enzymatic tyrosine sensor for quantification in human urine samples. Two fortified urine samples were analyzed, and the recovery values were 106 and 109%. The LOD and LOQ for tyrosine determination were 0.25 and 0.83 μmol L -1 , respectively, demonstrating that the proposed devices are suitable sensors for analyses of biological samples, even at low analyte concentrations.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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