Analyzing Blood Digestion: Estimation of Active Trypsin Levels in the Mosquito Midgut.
| Autor: | Reyes J; Department of Biochemistry and Molecular Biology, Veterinary, and Rangeland Sciences, University of Nevada, Reno, Nevada 89557, USA., Nuss A; Department of Biochemistry and Molecular Biology, Veterinary, and Rangeland Sciences, University of Nevada, Reno, Nevada 89557, USA.; Department of Agriculture, Veterinary, and Rangeland Sciences, University of Nevada, Reno, Nevada 89557, USA., Gulia-Nuss M; Department of Biochemistry and Molecular Biology, Veterinary, and Rangeland Sciences, University of Nevada, Reno, Nevada 89557, USA mgulianuss@unr.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2023 Apr 03; Vol. 2023 (4), pp. pdb.prot108224. Date of Electronic Publication: 2023 Apr 03. |
| DOI: | 10.1101/pdb.prot108224 |
| Abstrakt: | The N α-benzoyl-dl-arginine 4-nitroanilide hydrochloride (BApNA) assay is widely used to quantify trypsin in mosquito midguts and is highly sensitive. BApNA is a chromogenic substrate for proteolytic enzymes such as trypsin and amidase. Hydrolysis of BApNA at the bond between the arginine and the p-nitroaniline moieties releases the chromophore p-nitroaniline, which is detected by colorimetric analysis. The intensity of the color is directly proportional to the amount of trypsin in the solution. Here, we present a trypsin measurement assay specifically using the BApNA substrate. (© 2023 Cold Spring Harbor Laboratory Press.) |
| Databáze: | MEDLINE |
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