O-Glycomic and Proteomic Signatures of Spontaneous and Butyrate-Stimulated Colorectal Cancer Cell Line Differentiation.

Autor:	Madunić K; Center for Proteomics and Metabolomics, Leiden University, The Netherlands., Luijkx YMCA; Department Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands; Department Biomolecular Health Sciences, Utrecht University, Utrecht, The Netherlands., Mayboroda OA; Center for Proteomics and Metabolomics, Leiden University, The Netherlands., Janssen GMC; Center for Proteomics and Metabolomics, Leiden University, The Netherlands., van Veelen PA; Center for Proteomics and Metabolomics, Leiden University, The Netherlands., Strijbis K; Department Biomolecular Health Sciences, Utrecht University, Utrecht, The Netherlands., Wennekes T; Department Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands., Lageveen-Kammeijer GSM; Center for Proteomics and Metabolomics, Leiden University, The Netherlands., Wuhrer M; Center for Proteomics and Metabolomics, Leiden University, The Netherlands. Electronic address: m.wuhrer@lumc.nl.
Jazyk:	angličtina
Zdroj:	Molecular & cellular proteomics : MCP [Mol Cell Proteomics] 2023 Mar; Vol. 22 (3), pp. 100501. Date of Electronic Publication: 2023 Jan 18.
DOI:	10.1016/j.mcpro.2023.100501
Abstrakt:	Gut microbiota of the gastrointestinal tract provide health benefits to the human host via bacterial metabolites. Bacterial butyrate has beneficial effects on intestinal homeostasis and is the preferred energy source of intestinal epithelial cells, capable of inducing differentiation. It was previously observed that changes in the expression of specific proteins as well as protein glycosylation occur with differentiation. In this study, specific mucin O-glycans were identified that mark butyrate-induced epithelial differentiation of the intestinal cell line CaCo-2 (Cancer Coli-2), by applying porous graphitized carbon nano-liquid chromatography with electrospray ionization tandem mass spectrometry. Moreover, a quantitative proteomic approach was used to decipher changes in the cell proteome. It was found that the fully differentiated butyrate-stimulated cells are characterized by a higher expression of sialylated O-glycan structures, whereas fucosylation is downregulated with differentiation. By performing an integrative approach, we generated hypotheses about the origin of the observed O-glycome changes. These insights pave the way for future endeavors to study the dynamic O-glycosylation patterns in the gut, either produced via cellular biosynthesis or through the action of bacterial glycosidases as well as the functional role of these patterns in homeostasis and dysbiosis at the gut-microbiota interface. Competing Interests: Conflict of interest The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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