Caryocar brasiliense peel ethanolic extract has neuroprotective potential and reduces the activation of ERK1/2 in the ischemia and reperfusion brain acute phase in the rat.

Autor: Miguel MP; Programa de Pós Graduação em Ciência Animal da Escola de Veterinária e Zootecnia e Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Brazil. Electronic address: marinapacheco@ufg.br., de Menezes LB; Programa de Pós Graduação em Ciência Animal da Escola de Veterinária e Zootecnia e Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Brazil., Franco LG; Programa de Pós Graduação em Ciência Animal da Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Brazil., Andrascko MM; Animal Science graduated by Programa de Pós Graduação em Ciência Animal da Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Brazil., Parize ACB; Self-employed veterinarian, Brazil., de Almeida Borges JC; Animal Science graduated by Programa de Pós Graduação em Ciência Animal da Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Brazil., Guimarães LLB; Self-employed veterinarian, Brazil., Rezende E Silva D; Animal Science graduated by Programa de Pós Graduação em Ciência Animal da Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Brazil., Santos SDC; Instituto de Química, Universidade Federal de Goiás, Brazil., de Araújo EG; Programa de Pós Graduação em Ciência Animal da Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Brazil.
Jazyk: angličtina
Zdroj: Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association [J Stroke Cerebrovasc Dis] 2023 Mar; Vol. 32 (3), pp. 106945. Date of Electronic Publication: 2023 Jan 18.
DOI: 10.1016/j.jstrokecerebrovasdis.2022.106945
Abstrakt: Oxidative stress induced by ischemia and reperfusion (I/R) injury results in cell death by necrosis or apoptosis and triggers the activation of different intracellular pathways, such as mitogen-activated protein activated kinases. Pequi (Caryocar brasiliense) peel, residue of a fruit from Brazilian savannah-like vegetation, has phenolic compounds that have been demonstrated to have antioxidant effects in vitro. The present study aimed to evaluate the neuroprotective effects of C. brasiliense peel ethanolic extract (CBPE) against transient global I/R injury in the rat brain. Global ischemia for 5, 20, and 45 min followed by 2 h of reperfusion caused a significant time-dependent increase in the number of ischemic neurons (p ≤ 0.05); increased immunoreactivity of cleaved caspase-3 (CASP3); and activated extracellular signal-regulated kinase (ERK) 1/2. Pretreatment with CBPE (600 mg/kg, oral) or vitamin E (0.6 mg, oral) for 30 days showed significant protection against acute brain injury induced by 20 and 45 min or 5 min of ischemia, respectively, by reducing the cortical ischemic neuron count (p ≤ 0.05) and p-ERK1/2 immunoreactivity. In addition, active c-Jun N-terminal kinase (JNK) immunoreactivity was reduced in animals not subjected to ischemia. Therefore, we suggest an association between vitamin E and CBPE, which may generate a better neuroprotective response. Interestingly, mainly in the hippocampus and oligodendrocytes, high dose CBPE increase the number of isquemic neurons and of CASP3 immunoreactive cells in animals subjected or not to ischemia, which was not verified in the vitamin E group. Therefore, additional studies are recommended to verify the safety of the continuous use of CBPE.
Competing Interests: Conflicts of Interest The authors have no conflicts of interest to declare.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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