A Novel Trichothecene Toxin Phenotype Associated with Horizontal Gene Transfer and a Change in Gene Function in Fusarium .

Autor: Proctor RH; Mycotoxin Prevention and Applied Microbiology, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 1815 N University St., Peoria, IL 61604, USA., Hao G; Mycotoxin Prevention and Applied Microbiology, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 1815 N University St., Peoria, IL 61604, USA., Kim HS; Mycotoxin Prevention and Applied Microbiology, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 1815 N University St., Peoria, IL 61604, USA., Whitaker BK; Mycotoxin Prevention and Applied Microbiology, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 1815 N University St., Peoria, IL 61604, USA., Laraba I; Oak Ridge Institute for Science and Education, Mycotoxin Prevention and Applied Microbiology, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, Peoria, IL 61604, USA., Vaughan MM; Mycotoxin Prevention and Applied Microbiology, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 1815 N University St., Peoria, IL 61604, USA., McCormick SP; Mycotoxin Prevention and Applied Microbiology, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 1815 N University St., Peoria, IL 61604, USA.
Jazyk: angličtina
Zdroj: Toxins [Toxins (Basel)] 2022 Dec 24; Vol. 15 (1). Date of Electronic Publication: 2022 Dec 24.
DOI: 10.3390/toxins15010012
Abstrakt: Fusarium trichothecenes are among the mycotoxins of most concern to food and feed safety. Production of these mycotoxins and presence of the trichothecene biosynthetic gene ( TRI ) cluster have been confirmed in only two multispecies lineages of Fusarium : the Fusarium incarnatum - equiseti (Incarnatum) and F. sambucinum (Sambucinum) species complexes. Here, we identified and characterized a TRI cluster in a species that has not been formally described and is represented by Fusarium sp. NRRL 66739. This fungus is reported to be a member of a third Fusarium lineage: the F. buharicum species complex. Cultures of NRRL 66739 accumulated only two trichothecenes, 7-hydroxyisotrichodermin and 7-hydroxyisotrichodermol. Although these are not novel trichothecenes, the production profile of NRRL 66739 is novel, because in previous reports 7-hydroxyisotrichodermin and 7-hydroxyisotrichodermol were components of mixtures of 6-8 trichothecenes produced by several Fusarium species in Sambucinum. Heterologous expression analysis indicated that the TRI13 gene in NRRL 66739 confers trichothecene 7-hydroxylation. This contrasts the trichothecene 4-hydroxylation function of TRI13 in other Fusarium species. Phylogenetic analyses suggest that NRRL 66739 acquired the TRI cluster via horizontal gene transfer from a close relative of Incarnatum and Sambucinum. These findings provide insights into evolutionary processes that have shaped the distribution of trichothecene production among Fusarium species and the structural diversity of the toxins.
Databáze: MEDLINE
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