Different CprABC amino acid sequences affect nisin A susceptibility in Clostridioides difficile isolates.

Autor: Ide N; Department of General Dentistry, Hiroshima University Hospital, Hiroshima, Japan., Kawada-Matsuo M; Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan., Le MN; Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan., Hisatsune J; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan.; Antimicrobial Resistance Research Centre, National Institute of Infectious Diseases, Higashi Murayama, Japan., Nishi H; Department of General Dentistry, Hiroshima University Hospital, Hiroshima, Japan., Hara T; Antimicrobial Resistance Research Centre, National Institute of Infectious Diseases, Higashi Murayama, Japan.; Section of Clinical Laboratory, Division of Clinical Support, Hiroshima University Hospital, Hiroshima, Japan.; Division of Laboratory Medicine, Hiroshima University Hospital, Hiroshima, Japan., Kitamura N; Antimicrobial Resistance Research Centre, National Institute of Infectious Diseases, Higashi Murayama, Japan., Kashiyama S; Antimicrobial Resistance Research Centre, National Institute of Infectious Diseases, Higashi Murayama, Japan.; Section of Clinical Laboratory, Division of Clinical Support, Hiroshima University Hospital, Hiroshima, Japan.; Division of Laboratory Medicine, Hiroshima University Hospital, Hiroshima, Japan., Yokozaki M; Antimicrobial Resistance Research Centre, National Institute of Infectious Diseases, Higashi Murayama, Japan.; Division of Laboratory Medicine, Hiroshima University Hospital, Hiroshima, Japan., Kawaguchi H; Department of General Dentistry, Hiroshima University Hospital, Hiroshima, Japan., Ohge H; Antimicrobial Resistance Research Centre, National Institute of Infectious Diseases, Higashi Murayama, Japan.; Department of Infectious Diseases, Hiroshima University Hospital, Hiroshima, Japan., Sugai M; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan.; Antimicrobial Resistance Research Centre, National Institute of Infectious Diseases, Higashi Murayama, Japan., Komatsuzawa H; Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Jan 20; Vol. 18 (1), pp. e0280676. Date of Electronic Publication: 2023 Jan 20 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0280676
Abstrakt: Clinical isolates of Clostridioides difficile sometimes exhibit multidrug resistance and cause diarrhea after antibiotic administration. Metronidazole and vancomycin are often used as therapeutic agents, but resistance to these antibiotics has been found clinically. Therefore, the development of alternative antimicrobial agents is needed. Nisin A, produced by Lactococcus lactis, has been demonstrated to be effective against C. difficile infection. In this study, we evaluated the susceptibility of 11 C. difficile clinical isolates to nisin A and found that they could be divided into 2 groups: high and low susceptibility. Since CprABC and DltDABC, which are responsible for nisin A efflux and cell surface charge, respectively, have been reported to be related to nisin A susceptibility, we investigated the expression of cprA and dltA among the 11 strains. cprA expression in all strains was induced by nisin A, but dltA expression was not. The expression levels of both genes did not correlate with nisin A susceptibility in these clinical isolates. To evaluate cell surface charge, we performed a cytochrome C binding assay and found no relationship between charge and nisin A susceptibility. Then, we determined the whole genome sequence of each clinical isolate and carried out phylogenetic analysis. The 11 isolates separated into two major clusters, which were consistent with the differences in nisin A susceptibility. Furthermore, we found common differences in several amino acids in the sequences of CprA, CprB, and CprC between the two clusters. Therefore, we speculated that the different amino acid sequences of CprABC might be related to nisin A susceptibility. In addition, C. difficile strains could be divided in the same two groups based on susceptibility to epidermin and mutacin III, which are structurally similar to nisin A. These results suggest that genotypic variations in C. difficile strains confer different susceptibilities to bacteriocins.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Ide et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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