Cytochrome-c-oxidase and ATPsynthase content increases in the endometrium of the patients with adenomyosis.

Autor: Toniyan KA; Gynecology Department, FGBU KB1 (Volynskaya) UDP RF, 10, Starovolynskaya str, 121352, Moscow, Russia.; Cell Biophysics Laboratory, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe shosse, 123007, Moscow, Russia., Gorbacheva EY; Gynecology Department, FGBU KB1 (Volynskaya) UDP RF, 10, Starovolynskaya str, 121352, Moscow, Russia.; Cell Biophysics Laboratory, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe shosse, 123007, Moscow, Russia., Golubkova MA; Cell Biophysics Laboratory, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe shosse, 123007, Moscow, Russia., Povorova VV; Gynecology Department, FGBU KB1 (Volynskaya) UDP RF, 10, Starovolynskaya str, 121352, Moscow, Russia., Boyarintsev VV; Emergency and Extreme Medicine Department, FGBU DPO CGMA UDP RF, 19, Marshala Timoshenko str, 121359, Moscow, Russia., Ogneva IV; Cell Biophysics Laboratory, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe shosse, 123007, Moscow, Russia. iogneva@yandex.ru.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2023 Apr; Vol. 50 (4), pp. 3919-3925. Date of Electronic Publication: 2023 Jan 20.
DOI: 10.1007/s11033-023-08269-9
Abstrakt: Background: Adenomyosis is characterized by overgrowth of endometrial glands and stroma in the myometrium and is associated with reduced apoptosis. One of the key participants in one of the pathways of apoptosis is cytochrome c, whose expression can be regulated by actin-binding proteins involved in the formation of structures that provide cell motility. The aim of the study was to determine the content of actin-binding proteins, cytochrome c, and terminal members of the mitochondrial respiratory chain in endometrial biopsies of patients with adenomyosis and the control group.
Methods and Results: The content of all studied proteins was determined by Western blotting, and the mRNA content of the corresponding genes was determined by quantitative RT-PCR. The relative content of alpha-actinin1 and mRNA of the gene encoding it in biopsy specimens from patients with adenomyosis was higher than in controls by 86 and 84% (p < 0.05), respectively. The relative content of alpha-actinin4 did not change, as did cytochrome c. The content of cytochrome-c-oxidase and ATPsynthase in the group with adenomyosis exceeded the control level by 270 and 121% (p < 0.05), respectively, but the relative content of mRNA of these genes did not change, which may indicate a change in regulation at the level of protein synthesis.
Conclusion: The results may indicate a local increase in the synthesis of ATP in pathological endometrial cells, which indicates the possible effectiveness of local application of H+-ATP synthase inhibitors (for example, macrolide antibiotic) to reduce the severity of clinical symptoms of adenomyosis.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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