Cancer detection in clinical practice and using blood-based liquid biopsy: A retrospective audit of over 350 dogs.

Autor: Flory A; PetDx, Inc, La Jolla, California, USA., McLennan L; PetDx, Inc, La Jolla, California, USA., Peet B; PetDx, Inc, La Jolla, California, USA., Kroll M; PetDx, Inc, La Jolla, California, USA., Stuart D; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada., Brown D; Bridge Animal Referral Center, Edmonds, Washington, USA., Stuebner K; Clinical Investigation Center, University of Minnesota, Saint Paul, Minnesota, USA., Phillips B; Veterinary Specialty Hospital of San Diego, San Diego, California, USA., Coomber BL; Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada., Woods JP; Institute for Comparative Cancer Investigation at the Mona Campbell Centre for Animal Cancer, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada., Miller M; Veterinary Specialty Hospital of North County, San Marcos, California, USA., Tripp CD; Bridge Animal Referral Center, Edmonds, Washington, USA., Wolf-Ringwall A; Department of Veterinary Clinical Sciences, University of Minnesota, College of Veterinary Medicine, Saint Paul, Minnesota, USA., Kruglyak KM; PetDx, Inc, La Jolla, California, USA., McCleary-Wheeler AL; PetDx, Inc, La Jolla, California, USA., Phelps-Dunn A; PetDx, Inc, La Jolla, California, USA., Wong LK; PetDx, Inc, La Jolla, California, USA., Warren CD; PetDx, Inc, La Jolla, California, USA., Brandstetter G; PetDx, Inc, La Jolla, California, USA., Rosentel MC; PetDx, Inc, La Jolla, California, USA., DiMarzio LR; PetDx, Inc, La Jolla, California, USA., O'Kell AL; PetDx, Inc, La Jolla, California, USA., Cohen TA; PetDx, Inc, La Jolla, California, USA., Grosu DS; PetDx, Inc, La Jolla, California, USA., Chibuk J; PetDx, Inc, La Jolla, California, USA., Tsui DWY; PetDx, Inc, La Jolla, California, USA., Chorny I; PetDx, Inc, La Jolla, California, USA., Rafalko JM; PetDx, Inc, La Jolla, California, USA.
Jazyk: angličtina
Zdroj: Journal of veterinary internal medicine [J Vet Intern Med] 2023 Jan; Vol. 37 (1), pp. 258-267. Date of Electronic Publication: 2023 Jan 20.
DOI: 10.1111/jvim.16616
Abstrakt: Background: Guidelines-driven screening protocols for early cancer detection in dogs are lacking, and cancer often is detected at advanced stages.
Hypothesis/objectives: To examine how cancer typically is detected in dogs and whether the addition of a next-generation sequencing-based "liquid biopsy" test to a wellness visit has the potential to enhance cancer detection.
Animals: Client-owned dogs with definitive cancer diagnoses enrolled in a clinical validation study for a novel blood-based multicancer early detection test.
Methods: Retrospective medical record review was performed to establish the history and presenting complaint that ultimately led to a definitive cancer diagnosis. Blood samples were subjected to DNA extraction, library preparation, and next-generation sequencing. Sequencing data were analyzed using an internally developed bioinformatics pipeline to detect genomic alterations associated with the presence of cancer.
Results: In an unselected cohort of 359 cancer-diagnosed dogs, 4% of cases were detected during a wellness visit, 8% were detected incidentally, and 88% were detected after the owner reported clinical signs suggestive of cancer. Liquid biopsy detected disease in 54.7% (95% confidence interval [CI], 49.5%-59.8%) of patients, including 32% of dogs with early-stage cancer, 48% of preclinical dogs, and 84% of dogs with advanced-stage disease.
Conclusions/clinical Importance: Most cases of cancer were diagnosed after the onset of clinical signs; only 4% of dogs had cancer detected using the current standard of care (i.e., wellness visit). Liquid biopsy has the potential to increase detection of cancer when added to a dog's wellness visit.
(© 2023 PetDx, Inc and The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.)
Databáze: MEDLINE
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