Evaluation of drug-free methods for the detection of gene silencing in Saccharomyces cerevisiae .
| Autor: | Shaban K; Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada., Sauty SM; Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada., Fisher A; Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada., Cheng A; Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada., Yankulov K; Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Biochemistry and cell biology = Biochimie et biologie cellulaire [Biochem Cell Biol] 2023 Feb 01; Vol. 101 (1), pp. 125-130. Date of Electronic Publication: 2023 Jan 20. |
| DOI: | 10.1139/bcb-2022-0243 |
| Abstrakt: | Multiple studies in Saccharomyces cerevisiae have measured the levels of gene silencing by inserting the URA3 gene at various loci and selecting against URA3 -expressing cells by 5-flouroorotic acid (5-FOA). However, 5-FOA affects the cellular pools of dNTPs and can produce side effects. To circumvent this issue, we and others have introduced drug-free techniques to detect silent and active gene states. In this study, we compared three drug-free methods based on the expression of fluorescent reporters in the VIIL telomere of S. cerevisiae . Our results point out that only one of these methods is suitable for large-scale drug-free analyses of gene silencing. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|