Epitope tag-specific differences in the detection of COSA-1 marked crossover sites in C. elegans spermatocytes.
| Autor: | Cahoon CK; Institute of Molecular Biology, Department of Biology, University of Oregon, Eugene, OR, USA., Uebel CJ; Stanford University School of Medicine, Departments of Developmental Biology and Genetics, Stanford, CA, USA., Villeneuve AM; Stanford University School of Medicine, Departments of Developmental Biology and Genetics, Stanford, CA, USA., Libuda DE; Institute of Molecular Biology, Department of Biology, University of Oregon, Eugene, OR, USA. |
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| Jazyk: | angličtina |
| Zdroj: | MicroPublication biology [MicroPubl Biol] 2023 Jan 06; Vol. 2023. Date of Electronic Publication: 2023 Jan 06 (Print Publication: 2023). |
| DOI: | 10.17912/micropub.biology.000724 |
| Abstrakt: | Nascent crossover sites in C. elegans meiocytes can be cytologically detected using epitope-tagged versions of the pro-crossover protein COSA-1. In spermatocytes, differences exist between cytologically-detected and genetically-detected double crossover rates. Here, we examine nascent crossovers using both GFP- and OLLAS-tagged COSA-1. Similar to previous work, we find that most late pachytene spermatocytes display 5 COSA-1 foci, indicating one crossover per autosome bivalent. However, we detected more nuclei with >5 COSA-1 foci using OLLAS::COSA-1, reflecting some bivalents having 2 COSA-1 foci. These results demonstrate tag-specific differences in the detection of COSA-1 marked nascent crossovers in spermatocytes. (Copyright: © 2023 by the authors.) |
| Databáze: | MEDLINE |
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