Both male and female meiosis contribute to non-Mendelian inheritance of parental chromosomes in interspecific plant hybrids (Lolium × Festuca).

Autor: Majka J; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics, 77900, Olomouc, Czech Republic.; Institute of Plant Genetics, Polish Academy of Sciences, 60479, Poznan, Poland., Glombik M; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics, 77900, Olomouc, Czech Republic.; Department of Crop Genetics, John Innes Centre, Norwich. NR4 7UH, UK., Doležalová A; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics, 77900, Olomouc, Czech Republic., Kneřová J; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics, 77900, Olomouc, Czech Republic., Ferreira MTM; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics, 77900, Olomouc, Czech Republic.; Department of Biology, Federal University of Lavras, 37200-000, Lavras, MG, Brazil., Zwierzykowski Z; Institute of Plant Genetics, Polish Academy of Sciences, 60479, Poznan, Poland., Duchoslav M; Department of Botany, Palacký University, 77900, Olomouc, Czech Republic., Studer B; Molecular Plant Breeding, Institute of Agricultural Sciences, ETH Zurich, 8092, Zurich, Switzerland., Doležel J; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics, 77900, Olomouc, Czech Republic., Bartoš J; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics, 77900, Olomouc, Czech Republic., Kopecký D; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics, 77900, Olomouc, Czech Republic.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2023 Apr; Vol. 238 (2), pp. 624-636. Date of Electronic Publication: 2023 Feb 09.
DOI: 10.1111/nph.18753
Abstrakt: Some interspecific plant hybrids show unequal transmission of chromosomes from parental genomes to the successive generations. It has been suggested that this is due to a differential behavior of parental chromosomes during meiosis. However, underlying mechanism is unknown. We analyzed chromosome composition of the F 2 generation of Festuca × Lolium hybrids and reciprocal backcrosses to elucidate effects of male and female meiosis on the shift in parental genome composition. We studied male meiosis, including the attachment of chromosomes to the karyokinetic spindle and gene expression profiling of the kinetochore genes. We found that Lolium and Festuca homoeologues were transmitted differently to the F 2 generation. Female meiosis led to the replacement of Festuca chromosomes by their Lolium counterparts. In male meiosis, Festuca univalents were attached less frequently to microtubules than Lolium univalents, lagged in divisions and formed micronuclei, which were subsequently eliminated. Genome sequence analysis revealed a number of non-synonymous mutations between copies of the kinetochore genes from Festuca and Lolium genomes. Furthermore, we found that outer kinetochore proteins NDC80 and NNF1 were exclusively expressed from the Lolium allele. We hypothesize that silencing of Festuca alleles results in improper attachment of Festuca chromosomes to karyokinetic spindle and subsequently their gradual elimination.
(© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.)
Databáze: MEDLINE
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