| Autor: |
Hassan HA; Department of Botany and Microbiology, Faculty of Science, Minia University, Minia, Egypt., Abdelhafez OH; Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, New Minia City, Minia, Egypt., Shady NH; Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, New Minia City, Minia, Egypt., Yahia R; Department of Microbiology, Faculty of Pharmacy, Deraya University, New Minia City, Minia, Egypt., Mohamed NM; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Modern University for Technology and Information, Cairo, Egypt., Rateb ME; School of Computing, Engineering & Physical Sciences, University of the West of Scotland, Paisley, UK., Akil L; Strathclyde Institute of Pharmacy & Biomedical Sciences, University of Strathclyde, Glasgow, UK., Khadra I; Strathclyde Institute of Pharmacy & Biomedical Sciences, University of Strathclyde, Glasgow, UK., Glaeser SP; Institute of Applied Microbiology, Justus-Liebig University Gießen, Gießen, Germany., Kämpfer P; Institute of Applied Microbiology, Justus-Liebig University Gießen, Gießen, Germany., Abdelmohsen UR; Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, New Minia City, Minia, Egypt.; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt., El-Katatny MH; Department of Botany and Microbiology, Faculty of Science, Minia University, Minia, Egypt. |
| Abstrakt: |
Endophytic fungi are known to be a rich source of anti-infective drugs. In our study, Allium cepa was investigated for fungal diversity using different media to give 11 isolates which were identified morphologically. Out of the isolated fungal strains, Penicillium sp. (LCEF10) revealed potential anti-infective activity against the tested microbes ( Fusarium solani ATTC 25922, Pseudomonas aeruginosa (ATTC 29231), Staphylococcus aureus ATTC 27853 , Candida albicans ATTC 10231), besides, their MICs were measured by well diffusion method, therefore, it was subjected to molecular identification in addition to phylogenetic analysis. Moreover, the ITS sequence of strain LCEF10 showed a consistent assignment with the highest sequence similarity (99.81%) to Penicillium oxalicum NRRL 787. The crude ethyl acetate extract of Penicillium sp. LCEF10 was investigated for metabolomic analysis using LC-HR-ESI-MS. The metabolic profiling revealed the presence of polyketides, macrolides, phenolics and terpenoids. Furthermore, in silico molecular docking study was carried out to predict which compounds most likely responsible for the anti-infective activity. |
