Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigs.

Autor: Ranjitha HB; FMD Vaccine Research Laboratory, ICAR-Indian Veterinary Research Institute, Hebbal, Bengaluru, 560 024, India., Dhanesh VV; FMD Vaccine Research Laboratory, ICAR-Indian Veterinary Research Institute, Hebbal, Bengaluru, 560 024, India., Hosamani M; FMD Vaccine Research Laboratory, ICAR-Indian Veterinary Research Institute, Hebbal, Bengaluru, 560 024, India., Sreenivasa BP; FMD Vaccine Research Laboratory, ICAR-Indian Veterinary Research Institute, Hebbal, Bengaluru, 560 024, India., Jabeen U; FMD Vaccine Research Laboratory, ICAR-Indian Veterinary Research Institute, Hebbal, Bengaluru, 560 024, India., Biswal JK; Directorate of Foot and Mouth Disease, ICAR-International Centre for FMD, Bhubaneswar, 752 050, India., Saravanan P; FMD Vaccine Research Laboratory, ICAR-Indian Veterinary Research Institute, Hebbal, Bengaluru, 560 024, India., Sanyal A; FMD Vaccine Research Laboratory, ICAR-Indian Veterinary Research Institute, Hebbal, Bengaluru, 560 024, India., Bhanuprakash V; FMD Vaccine Research Laboratory, ICAR-Indian Veterinary Research Institute, Hebbal, Bengaluru, 560 024, India., Basagoudanavar SH; FMD Vaccine Research Laboratory, ICAR-Indian Veterinary Research Institute, Hebbal, Bengaluru, 560 024, India. basagoudanavar.sh@icar.gov.in.
Jazyk: angličtina
Zdroj: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2023 Feb; Vol. 107 (4), pp. 1285-1297. Date of Electronic Publication: 2023 Jan 19.
DOI: 10.1007/s00253-023-12359-w
Abstrakt: Foot-and-mouth disease (FMD) is a contagious viral disease of high economic importance, caused by FMD virus (FMDV), a positive-sense single-stranded RNA virus, affecting cloven-hoofed animals. Preventive vaccination using inactivated virus is in practice to control the disease in many endemic countries. While the vaccination induces antibodies mainly to structural proteins, the presence of antibodies to the non-structural proteins (NSP) is suggestive of infection, a criterion for differentiation of infected from vaccinated animals (DIVA). Also, there is a growing demand for enhancing the stability of the FMD vaccine virus capsid antigen as the strength of the immune response is proportional to the amount of intact 146S particles in the vaccine. Considering the need for a DIVA compliant stable vaccine, here we report generation and rescue of a thermostable and negative marker virus FMDV serotype O (IND/R2/1975) containing a partial deletion in non-structural protein 3A, generated by reverse genetics approach. Immunization of guinea pigs with the inactivated thermostable-negative marker virus antigen induced 91% protective immune response. Additionally, a companion competitive ELISA (cELISA) targeting the deleted 3A region was developed, which showed 92.3% sensitivity and 97% specificity, at cut-off value of 36% percent inhibition. The novel thermostable-negative marker FMDV serotype O vaccine strain and the companion cELISA could be useful in FMDV serotype O enzootic countries to benefit the FMD control program. KEY POINTS: • Thermostable foot-and-mouth disease virus serotype O with partial deletion in 3A. • Inactivated thermostable marker vaccine induced 91% protection in guinea pigs. • Companion cELISA based on deleted region in 3A could potentially facilitate DIVA.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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