l-Theanine Goes Greener: A Highly Efficient Bioprocess Catalyzed by the Immobilized γ-Glutamyl Transferase from Bacillus subtilis.

Autor: Robescu MS; Department of Drug Sciences, University of Pavia, viale Taramelli 12, Pavia, Italy., Alcántara AR; Department of Chemistry in Pharmaceutical Sciences, Complutense University of Madrid, Plaza de Ramon y Cajal s/n, Madrid, Spain., Calvio C; Department of Biology and Biotechnology, University of Pavia, via Ferrata 1, Pavia, Italy., Morelli CF; Department of Chemistry, University of Milan, via Golgi 19, Milano, Italy., Speranza G; Department of Chemistry, University of Milan, via Golgi 19, Milano, Italy., Ubiali D; Department of Drug Sciences, University of Pavia, viale Taramelli 12, Pavia, Italy., Bavaro T; Department of Drug Sciences, University of Pavia, viale Taramelli 12, Pavia, Italy.
Jazyk: angličtina
Zdroj: ChemSusChem [ChemSusChem] 2023 Apr 21; Vol. 16 (8), pp. e202202108. Date of Electronic Publication: 2023 Mar 13.
DOI: 10.1002/cssc.202202108
Abstrakt: l-Theanine (l-Th) was synthesized by simply mixing the reactants (l-glutamine and ethylamine in water) at 25 °C and Bacillus subtilis γ-glutamyl transferase (BsGGT) covalently immobilized on glyoxyl-agarose according to a methodology previously reported by our research group; neither buffers, nor other additives were needed. Ratio of l-glutamine (donor) to ethylamine (acceptor), pH, enzymatic units (IU), and reaction time were optimized (molar ratio of donor/acceptor=1 : 8, pH 11.6, 1 IU mL -1 , 6 h), furnishing l-Th in 93 % isolated yield (485 mg, 32.3 g L -1 ) and high purity (99 %), after a simple filtration of the immobilized biocatalyst, distillation of the volatiles (unreacted ethylamine) and direct lyophilization. Immobilized BsGGT was re-used (four reaction cycles) with 100 % activity retention. This enzymatic synthesis represents a straightforward, fast, high-yielding, and easily scalable approach to l-Th preparation, besides having a favorable green chemistry metrics.
(© 2023 The Authors. ChemSusChem published by Wiley-VCH GmbH.)
Databáze: MEDLINE
Externí odkaz: