The FANCC-FANCE-FANCF complex is evolutionarily conserved and regulates meiotic recombination.

Autor:	Singh DK; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany., Gamboa RS; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany., Singh AK; CSIR-Centre for Cellular & Molecular Biology, Uppal Road, Hyderabad 500007, India., Walkemeier B; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany., Van Leene J; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent B-9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent B-9052, Belgium., De Jaeger G; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent B-9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent B-9052, Belgium., Siddiqi I; CSIR-Centre for Cellular & Molecular Biology, Uppal Road, Hyderabad 500007, India., Guerois R; Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette 91190, France., Crismani W; The DNA Repair and Recombination Laboratory, St Vincent's Institute of Medical Research, Melbourne 3065, Australia.; The Faculty of Medicine, Dentistry and Health Science, The University of Melbourne, Parkville, Victoria, Australia., Mercier R; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany.
Jazyk:	angličtina
Zdroj:	Nucleic acids research [Nucleic Acids Res] 2023 Apr 11; Vol. 51 (6), pp. 2516-2528.
DOI:	10.1093/nar/gkac1244
Abstrakt:	At meiosis, programmed meiotic DNA double-strand breaks are repaired via homologous recombination, resulting in crossovers (COs). From a large excess of DNA double-strand breaks that are formed, only a small proportion gets converted into COs because of active mechanisms that restrict CO formation. The Fanconi anemia (FA) complex proteins AtFANCM, MHF1 and MHF2 were previously identified in a genetic screen as anti-CO factors that function during meiosis in Arabidopsis thaliana. Here, pursuing the same screen, we identify FANCC as a new anti-CO gene. FANCC was previously only identified in mammals because of low primary sequence conservation. We show that FANCC, and its physical interaction with FANCE-FANCF, is conserved from vertebrates to plants. Further, we show that FANCC, together with its subcomplex partners FANCE and FANCF, regulates meiotic recombination. Mutations of any of these three genes partially rescues CO-defective mutants, which is particularly marked in female meiosis. Functional loss of FANCC, FANCE, or FANCF results in synthetic meiotic catastrophe with the pro-CO factor MUS81. This work reveals that FANCC is conserved outside mammals and has an anti-CO role during meiosis together with FANCE and FANCF. (© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.)
Databáze:	MEDLINE
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