| Autor: |
Palanisamy DS; Bharathiar University, Research and Development Centre, Coimbatore, India.; Nandha Arts and Science College, Department of Biotechnology, Erode, India., Gounder BS; Government Arts College, Department of Botany, Salem, India., Selvaraj K; Bharathiar University, Research and Development Centre, Coimbatore, India.; Nandha Arts and Science College, Department of Biotechnology, Erode, India., Kandhasamy S; Nandha Arts and Science College, Department of Biotechnology, Erode, India., Alqahtani T; King Khalid University, College of Pharmacy, Department of Pharmacology, Abha, Saudi Arabia., Alqahtani A; King Khalid University, College of Pharmacy, Department of Pharmacology, Abha, Saudi Arabia., Chidambaram K; King Khalid University, College of Pharmacy, Department of Pharmacology, Abha, Saudi Arabia., Arunachalam K; Mulungushi University, School of Science, Engineering and Technology, Department of Science and Mathematics, Kabwe, Zambia., Alkahtani AM; King Khalid University, College of Medicine, Department of Microbiology & Clinical Parasitology, Abha, Saudi Arabia., Chandramoorthy HC; King Khalid University, College of Medicine, Department of Microbiology & Clinical Parasitology, Abha, Saudi Arabia.; King Khalid University, College of Medicine, Centre for Stem Cell Research, Abha, Saudi Arabia., Sharma N; Maharishi Markandeshwar University, Research and Development Centre, Solan, Himachal Pradesh, India., Rajeshkumar S; Saveetha Institute of Medical and Technical Sciences, Department of Pharmacology, Chennai, India., Marwaha L; Lovely Professional University, School of Bioengineering and Bio Sciences, Department of Zoology, Punjab, India. |
| Abstrakt: |
Silver nanoparticles are opted to have various applications in different fields ranging from traditional medicines to culinary items. It is toxic and most effective against bacteria, fungi viruses, parasites, parasite carrying vectors such as mosquitoes and their larvae and other eukaryotic microorganisms at low concentration without any side effects and toxicity to humans. In view of these data, the present research has been investigated by synthesizing silver nanoparticles using 1mM silver nitrate and aqueous extract of Passiflora foetida. The variation of nanoparticles in size and shape concerning the concentration of extract prepared were analysed. The formation of silver nanoparticles was confirmed by colour changing from yellowish green to reddish-brown implicating the surface plasmon resonance. Further, it was concluded by obtaining an absorbance peak at 420 nm using UV-Visible spectrophotometer analysis. FTIR analysis was used to identify the capping ligands, which included alkanes, aromatic groups and nitro compounds. The average grain size of ~12 nm to 14 nm with crystalline phase was revealed by X-ray Diffraction studies. The SEM images depicted the surface morphology with agglomeration; TEM studies showed the shape of nanoparticles as spherical and hexagonal with sizes ranging from 40 nm to 100 nm and EDAX analysis confirmed the presence of elemental silver as the principal constituent. The characterized silver nanoparticles were then tested for synergistic antibacterial effects with tetracycline, and the results show that they are more active against E. coli and S. aureus, but moderately effective against B. cereus and K. pneumoniae . It also had a strong larval and pupal toxic effects on the dengue vector, Aedes aegypti with the highest mortality. As a result, silver nanoparticles could be a viable alternative for a variety of applications. |
