Enhancement of polyethylene glycol-cell fusion efficiency by novel application of transient pressure using a jet injector.

Autor: Chang CY; Department of Device Application for Molecular Therapeutics, Graduate School of Medicine, Osaka University, Japan., Tai JA; Department of Device Application for Molecular Therapeutics, Graduate School of Medicine, Osaka University, Japan., Sakaguchi Y; Medical Device Division, Industry Business Unit, Safety Strategic Business Unit, Daicel Co., Osaka, Japan., Nishikawa T; Department of Device Application for Molecular Therapeutics, Graduate School of Medicine, Osaka University, Japan., Hirayama Y; Medical Device Division, Industry Business Unit, Safety Strategic Business Unit, Daicel Co., Osaka, Japan., Yamashita K; Department of Device Application for Molecular Therapeutics, Graduate School of Medicine, Osaka University, Japan.; Medical Device Division, Industry Business Unit, Safety Strategic Business Unit, Daicel Co., Osaka, Japan.; Medical Device Development, Medical Device Division, Industry Business Unit, Safety Strategic Business Unit, Daicel Co., Osaka, Japan.
Jazyk: angličtina
Zdroj: FEBS open bio [FEBS Open Bio] 2023 Mar; Vol. 13 (3), pp. 478-489. Date of Electronic Publication: 2023 Jan 27.
DOI: 10.1002/2211-5463.13557
Abstrakt: Cell-cell fusion involves the fusion of somatic cells into a single hybrid cell. It is not only a physiological process but also an important cell engineering technology which can be applied to various fields, such as regenerative medicine, antibody engineering, genetic engineering, and cancer therapy. There are three major methods of cell fusion: electrical cell fusion, polyethylene glycol (PEG) cell fusion, and virus-mediated cell fusion. Although PEG cell fusion is the most economical approach and does not require expensive instrumentation, it has a poor fusion rate and induces a high rate of cell cytotoxicity. To improve the fusion rate of the PEG method, we combined it with the pyro-drive jet injector (PJI). PJI provides instant pressure instead of cell agitation to increase the probability of cell-to-cell contact and shorten the distance between cells in the process of cell fusion. Here, we report that this improved fusion method not only decreased cell cytotoxicity during the fusion process, but also increased fusion rate compared with the conventional PEG method. Furthermore, we tested the functionality of cells fused using the PJI-PEG method and found them to be comparable to those fused using the conventional PEG method in terms of their application for dendritic cell (DC)-tumor cell fusion vaccine production; in addition, the PJI-PEG method demonstrated excellent performance in hybridoma cell preparation. Taken together, our data indicate that this method improves cell fusion efficiency as compared to the PEG method and thus has the potential for use in various applications that require cell fusion technology.
(© 2023 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
Databáze: MEDLINE
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