Moderate but not severe hypothermia increases intracellular cyclic AMP through preserved production and reduced elimination.

Autor: Kuzmiszyn AK; Experimental and Clinical Pharmacology, Department of Medical Biology, UiT - the Arctic University of Norway, Tromsø, Norway; Norwegian Air Ambulance Foundation, Research and Development Department, Oslo, Norway; Division of Surgical Medicine and Intensive Care, University Hospital of North Norway, Tromsø, Norway., Selli AL; Experimental and Clinical Pharmacology, Department of Medical Biology, UiT - the Arctic University of Norway, Tromsø, Norway., Furuholmen M; Experimental and Clinical Pharmacology, Department of Medical Biology, UiT - the Arctic University of Norway, Tromsø, Norway., Smaglyukova N; Experimental and Clinical Pharmacology, Department of Medical Biology, UiT - the Arctic University of Norway, Tromsø, Norway., Kondratiev T; Anesthesia and Critical Care Research Group, Department of Clinical Medicine, UiT - the Arctic University of Norway, Tromsø, Norway., Fuskevåg OM; Department of Laboratory Medicine, Division of Diagnostic Services, University Hospital of North Norway, Tromsø, Norway., Sager G; Experimental and Clinical Pharmacology, Department of Medical Biology, UiT - the Arctic University of Norway, Tromsø, Norway., Dietrichs ES; Experimental and Clinical Pharmacology, Department of Medical Biology, UiT - the Arctic University of Norway, Tromsø, Norway; Center for Psychopharmacology, Diakonhjemmet Hospital, Oslo, Norway. Electronic address: erik.sveberg.dietrichs@uit.no.
Jazyk: angličtina
Zdroj: Cryobiology [Cryobiology] 2023 Mar; Vol. 110, pp. 18-23. Date of Electronic Publication: 2023 Jan 14.
DOI: 10.1016/j.cryobiol.2023.01.005
Abstrakt: Rewarming from accidental hypothermia could be complicated by acute cardiac dysfunction but providing supportive pharmacotherapy at low core temperatures is challenging. Several pharmacological strategies aim to improve cardiovascular function by increasing cAMP in cardiomyocytes as well as cAMP and cGMP levels in vascular smooth muscle, but it is not clear what effects temperature has on cellular elimination of cAMP and cGMP. We therefore studied the effects of differential temperatures from normothermia to deep hypothermia (37 °C-20 °C) on cAMP levels in embryonic H9c2 cardiac cells and elimination of cAMP and cGMP by PDE-enzymes and ABC-transporter proteins. Our experiments showed significant elevation of intracellular cAMP in H9c2-cells at 30 °C but not 20 °C. Elimination of both cAMP and cGMP through ABC transport-proteins and PDE-enzymes showed a temperature dependent reduction. Accordingly, the increased cardiomyocyte cAMP-levels during moderate hypothermia appears an effect of preserved production and reduced elimination at 30 °C. This correlates with earlier in vivo findings of a positive inotropic effect of moderate hypothermia.
(Copyright © 2023. Published by Elsevier Inc.)
Databáze: MEDLINE
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